Figure 2. Inhibition of Slpr suppresses the phenotypes induced by Src activation.

(A) Src42A constitutively active (CA) expression in the wing disc induces organismal lethality, which is suppressed by knockdown of slpr. One-way ANOVA with Sidak’s post-test. (B) The small, disheveled wing phenotype of the rare escapers with Src42A CA is suppressed by knockdown of slpr. (C) Src42A CA-mediated JNK activation, which was detected by the TRE-RFP reporter, is suppressed by knockdown of slpr. (D) Quantification of TRE-RFP in C. One-way ANOVA with Sidak’s post-test. Scale bars, 100 µm.

Figure 2—figure supplement 1. Inhibition of Slpr suppresses the phenotypes induced by Src activation.

(A) In the first screening, 107 RNAi stocks were used. The numbers of survivors were counted. The blue color indicates that survivors have disheveled wings while the magenta color indicates that survivors have the recovered wing phenotype. (B) In the second screening, the RNAi stocks that produced more than two survivors in the first screening were used. The blue color indicates that survivors have disheveled wings while the magenta color indicates that survivors have the recovered wing phenotype. (C) RT-qPCR of slpr using RNA from the larval whole body. slpr RNAi BL32948 lowered the transcriptional expression of slpr. (D) Src42A expression in the eye imaginal disc with gmr-Gal4 eliminates the adult eye, which can be suppressed by knockdown of slpr. Note that the disheveled wing phenotype induced by leaky expression of gmr-Gal4 in the wing disc is also suppressed by knockdown of slpr. (E) Src42A expression in the eye disc induces organismal lethality during development, which is suppressed by slpr knockdown. Two-tailed unpaired t-test.