Fig. S2.

Effects of CFI-400945 and Radiation on Organoid Formation in Breast Cancer Patient-Derived Organoids. Quality assurance for mouse cell contamination in BXTO64 (A.) and BXTO81 (B.) organoid models established from patient-derived mouse xenograft (PDX) tissue (see Supplementary Table 1). Established BXTO64 and BXTO81 organoids were stained with mouse anti-H2K-FITC and anti-human EpCAM-APC and analyzed by flow cytometry for mouse and human cell content, respectively, showing that at least ∼95% of the preparation contains human cancer cells as shown. Effect of CFI-400945 and radiation in BXTO64 (C.) and BXTO81 (D.) models. Average number of organoids was normalized to that of control (no-radiation, no-drug). Averages and standard deviations (SD, bars) of duplicate experiments are presented.