SPOP targets oncogenic protein ZBTB3 for destruction to suppress endometrial cancer

We recently found a mistake in Figure 5C and 5D, the pictures of colony formation of “sh-ZBTB3#1” in the Figure 5C and “EV+sh-ZBTB3#1” in the Figure 5D were misused. The corrected Figure 5D is shown below. The authors declare that this correction does not change the results or conclusions of this paper.

Figure 5.

SPOP suppresses cell proliferation, migration and invasion partially dependent on ZBTB3. (A) Western blot (left panel) and Cell proliferation assay (right panel) of ECC-1 cells infected with lentivirus expressing the indicated shRNAs. Standard deviation (S.D.) of at least three independent experiments is shown to indicate statistical significance. *P < 0.05. (B) Western blot (left panel) and Cell proliferation assay (right panel) of ECC-1 cells infected with empty vector or lentivirus expressing FLAG-SPOP-G75R in combination with control shRNA or ZBTB3-specific shRNAs. Data are shown as means ± SD (n=3). *P < 0.05. (C) Cell colony formation assay of ECC-1 cells infected with lentivirus expressing the indicated shRNAs. All data shown are mean values ± SD from three replicates. *P < 0.05. (D) Cell colony formation assay of ECC-1 cells infected with empty vector or lentivirus expressing FLAG-SPOP-G75R in combination with control shRNA or ZBTB3-specific shRNAs. Cell migration (E) and invasion (F) assay of ECC-1 cells infected with lentivirus expressing the indicated shRNAs. Data are shown as means ± SD (n=3). *P < 0.05. (G, H) Cell migration (G) and invasion (H) assay of ECC-1 cells with lentivirus expressing FLAG-SPOP-G75R in combination with control shRNA or ZBTB3-specific shRNAs. Data are shown as means ± SD (n=3). *P < 0.05.