Figure 1.

Overview of tissue clearing methods used on heart

Tissue clearing methods are divided into four groups based on their clearing principle. Solvent-based (yellow), simple immersion (green), hyperhydration (purple), and hydrogel embedding (red). Applicability of each method is marked in a dark color: GFP, method preserves natural GFP fluorescence; IHC, method can be used with immunohistochemistry; DAPI, method is suitable for DAPI/Hoechst nuclei staining. Light color, method is not suitable for this methodology. To summarize, most of the methods used on heart clearing are applicable for many techniques.