Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 1;24(4):102387. doi: 10.1016/j.isci.2021.102387

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Workflow for 3D imaging in adult mouse heart

(A) Custom made imaging chamber with pinned embryonic heart for 2x lens imaging. Glass tubes border the chamber and support the glass coverslip.

(B) Imaging of the heart on an upright confocal microscope with immersion objective lens 25×; the lens is immersed in a chamber filled up with SCALE with the heart specimen pinned at the bottom.

(C) Field of view using 2x lens, allowing visualization of the entire mouse heart (postnatal day 30) without image stitching. The highlighted region was imaged also by the 10× objective to obtain a high-resolution 3D dataset of the left bundle branch. Cx40-GFP (green), tissue autofluorescence (red).

(D and E) Left ventricular lateral wall imaged with 4x dry lens for overview of the Purkinje network on Cx40-GFP mouse (D) with one region (boxed area) imaged with 25x ScaleView lens (E).

Depth of imaging could be appreciated on orthogonal views below, allowing clear distinction between subendocardial Purkinje fibers (solid bands) and more deeply located coronary artery branch (arrow) (F).