Table 1.
Tissue clearing methods used on heart tissue—abbreviations and names
Method name | Full name of the method | Citation | |
---|---|---|---|
Solvent based | BABB | Benzyl-Alcohol:Benzyl-Benzoate | Zucker et al., (1998) |
FluorClear BABB | Fluorophore preservation Clearing Benzyl-Alcohol:Benzyl-Benzoate | Schwarz et al. (2015); Foster et al. (2019) | |
DBE | Phenylmethoxymethylbenzene (Dibenzyl Ether) | Becker et al., (2012) | |
3DISCO | Three-Dimensional Imaging of Solvent-Cleared Organs | Molbay et al., (2021) | |
iDISCO | Immunolabeling-enabled three-Dimensional Imaging of Solvent-Cleared Organs | Renier et al., (2016) | |
Ethanol-Eci | Ethanol-ethyl-3-phenylprop-2-enoate (Ethyl Cinnamate [ECi]) | Klingberg et al. (2017) | |
FASTClear | Free of Acrylamide SDS-based Tissue Clearing | Liu et al., (2017) | |
Simple immersion | FRUIT | Fructose and Urea | Hou et al., (2015) |
MACS | MXDA-based Aqueous Clearing System | Zhu et al. (2020) | |
SeeDB | See Deep Brain | Ke et al. (2013); Murray et al. (2015) | |
Sucrose | Sucrose | Tsai et al., (2009) | |
Hyperhydratation | CUBIC | Clear, Unobstructed Brain Imaging Cocktails and computational analysis | Susaki et al. (2014); Susaki et al., (2015) |
SCALE S | SCALE—name, not abbreviation | Hama et al. (2015) | |
SCALE A2 | SCALE—name, not abbreviation | Hama et al. (2015) | |
SUT | Scheme Update on tissue Transparency | Wang et al. (2018); Matryba et al. (2019) | |
Hydrogel embedding | ACT-PRESTO | Active Clarity Technique-Pressure Related Efficient and Stable Transfer of macromolecules into Organs technique | Lee et al. (2016) |
Clarity | Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/in situ-hybridization-compatible Tissue hYdrogel | Chung et al. (2013); Tomer et al. (2014) | |
SCM | Simplified Clarity Method | Sung et al. (2016); Matryba et al. (2019) | |
PACT | PAssive Clarity Technique | Yang et al., (2014); Neckel et al., (2016) | |
PARS | Perfusion-assisted Agent Release in Situ | Yang et al., (2014) | |
SWITCH | System-Wide control of Interaction Time and kinetics of Chemicals | Murray et al. (2015) |