FIG 4.

Effect of mutations in C. dubliniensis orthologs of S. cerevisiae spore wall genes on the chlamydospore wall. (A) Cells of strains of the indicated genotype were grown on SGlycerol medium and then stained with both Eosin Y to label chitosan and Calcofluor White (CFW) to label chitin or chitosan. Arrowheads indicate examples of chlamydospores visible in the images. Arrows indicate examples of CFW-stained septa. Scale bar, 10 μm. (B) The intensity of the Eosin Y fluorescence was categorized as bright, dim, or no fluorescence for each chlamydospore, and the number of chlamydospores in each category for each strain was quantified. For each strain, the value represents the average for 100 chlamydospores in each of three independent experiments. Error bars indicate one standard deviation. One asterisk (*) indicates significant difference at P < 0.05; two asterisks (**) indicates significant difference at P < 0.0005 (Student t test).