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. 2021 Mar 30;12(2):e00256-21. doi: 10.1128/mBio.00256-21

FIG 11.

FIG 11

PEDV fusion. (A) Time-lapse images of PEDV (red) fused in Rab5-positive early endosome (green). Scale bar, 2 μm. (B) Time-lapse Rab5-EGFP (green), Rab7 (white), and DiD-labeled PEDV (red) fluorescence intensities corresponding to A. (C) Time-lapse images of PEDV (red) fused in Rab5 (green) and Rab7 (white) double-positive endosome. Scale bar, 2 μm. (D) Time-lapse Rab5 (green), Rab7 (blue,) and DiD-labeled PEDV (red) fluorescence intensities corresponding to C. (E) Time-lapse images of PEDV (red) fused in Rab7-positive late endosome (white). Scale bar, 2 μm. (F) Time-lapse Rab5 (green), Rab7 (blue), and DiD-labeled PEDV (red) fluorescence intensities corresponding to E. (G) Proportion of PEDV fusion with Rab5-positive (n = 6), Rab5 and Rab7 double positive (n = 8), and Rab7-positive (n = 34) endosomes. (H) Colocalization time of PEDV with Rab5-positive, Rab5 and Rab7 double positive, and Rab7-positive endosomes before viral fusion. Error bars represent SD.