TABLE 1.
Phenotypic characterization of relevant strainsa
| Strain | Description of strain | Mean growth rate constant (μ) ± SD | Mean cell length (μm) ± SD | % Minicells |
|---|---|---|---|---|
| 168 | Wild type | 0.53 ± 0.004 | 3.11 ± 0.77 | 0.3 |
| 3309 | ΔminCD | 0.45 ± 0.021 | 7.64 ± 2.70 | 45.8 |
| RD021 | ΔminJ | 0.51 ± 0.049 | 6.65 ± 2.02 | 13.8 |
| 4041 | ΔdivIVA | 0.46 ± 0.020 | 8.13 ± 3.40 | 29.6 |
| BHF011 | Dendra2-MinD | 0.49 ± 0.004 | 2.67 ± 0.61 | 0.9 |
| BHF017 | msfGFP-MinD | 0.55 ± 0.004 | 4.22 ± 1.04 | 9.1 |
| JB38 | MinJ-Dendra2 | 0.51 ± 0.006 | 3.44 ± 1.06 | 0 |
| BHF007 | MinJ-msfGFP | 0.57 ± 0.013 | 3.38 ± 0.76 | 0.3 |
| JB40 | MinJ-mNeonGreen | 0.57 ± 0.002 | 3.16 ± 0.67 | 0 |
| JB36 | DivIVA-Dendra2 | 0.50 ± 0.007 | 4.33 ± 0.92 | 8.0 |
| 1803 | DivIVA-GFP | 0.45 ± 0.021 | 3.31 ± 0.73 | 1.1 |
| BHF028 | DivIVA-mNeonGreen | 0.54 ± 0.029 | 5.42 ± 1.35 | 5.3 |
| JB37 | DivIVA-PAmCherry | 0.51 ± 0.019 | 4.35 ± 1.11 | 3.3 |
a
For determination of the growth rate constant, μ, the optical density at 600 nm of exponentially growing cells was measured. Cell length and the percentage of minicells were determined microscopically using Fiji, with n ≥ 200. Strains were grown in independent triplicates, with differences reflected in the standard deviation (SD).