TABLE 3.
Results of FRAP analysis for Min proteins in different genetic backgrounds
| Protein and genetic background | Fluorophore | Diffusion coefficient (μm² · 10−3 · s−1) | Half-time recovery (s) | Mobile fraction |
|---|---|---|---|---|
| MinD in wild type | msfGFP | 57.8 ± 10.1 | 7.55 ± 1.31 | 0.79 |
| MinJ in wild type | msfGFP | 7.19 ± 2.27 | 62.4 ± 19.7 | 0.77 |
| MinJ in ΔminCD mutant | msfGFP | 14.5 ± 9.54 | 30.2 ± 19.9 | 0.75 |
| DivIVA in wild type | GFP | 3.39 ± 0.82 | 128 ± 30.9 | 0.65 |
| DivIVA in ΔminCD mutant | GFP | 3.74 ± 1.36 | 116 ± 42.4 | 0.68 |
| DivIVA in ΔminJ mutant | GFP | 8.57 ± 4.43 | 50.9 ± 26.4 | 0.49 |
| DivIVA in ΔminCDJ mutant | GFP | 4.98 ± 2.93 | 87.7 ± 51.6 | 0.61 |
| DivIVA in wild type | mNeonGreen | 7.23 ± 1.99 | 60.3 ± 16.6 | 0.64 |
| DivIVA in ΔminCD mutant | mNeonGreen | 6.88 ± 2.76 | 63.4 ± 25.4 | 0.67 |
| DivIVA in ΔminJ mutant | mNeonGreen | 18.0 ± 3.22 | 24.4 ± 4.33 | 0.39 |
| DivIVA in ΔminCDJ mutant | mNeonGreen | 9.47 ± 4.26 | 46.1 ± 20.7 | 0.66 |