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. 2021 Apr 27;12(2):e00567-21. doi: 10.1128/mBio.00567-21

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Copyright © 2021 Fu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 11 — (A to C) The survival of parent strains and isolates in culture with BMDMs. The survival of (A) H99, (B) A1-35-8, and (C) Ftc555-1 isolates was determined by CFU after 0 and 24 h phagocytosis. The percentage of survival was calculated by normalizing the CFU value of 24 h infection to time zero. HP, AP, FP: parental strains; HC, AC, FC: controls Data represent the mean of three biological replicates, and error bars are SD. There was no statistically significant difference between isolates and their parental strains as determined by one-way ANOVA followed by Tukey’s multiple-comparison test. (D) BMDMs were infected with Ftc555-1 isolates for 48 h. Lactate dehydrogenase (LDH) release from damaged BMDMs into culture supernatant was assayed. ***, P < 0.001; one-way ANOVA followed by Tukey’s multiple-comparison test.