FIG 4.
Growth in mouse serum elicits increased capsule thickness and cell body diameter in the pdr802 mutant. (A) Light micrographs of the indicated strains after growth in mouse serum (at 37°C, 5% CO2) for 24 h and negative staining with India ink to visualize the capsule. All images are to the same scale; bar, 5 μm. (B) Mean and SD of capsule thickness, assessed by measuring at least 50 cells per strain with ImageJ. (C) Cells grown as described for panel A were plated on YPD to assess CFU. Mean and SD of the fold change compared to 0 h are shown. (D) Mean and SD of cell body diameter, measured as for panel B. ***, P < 0.001, and ****, P < 0.0001, compared to KN99α by one-way ANOVA with a post hoc Dunnett test.