FIG 4.

The combination of METH and sucrose increases water (H₂O) consumption by C57BL/6 mice. The animals’ total H₂O consumption after 21 days of METH injection and sucrose administration is shown. Mice were daily injected intraperitoneally with PBS (untreated) or METH (2.5, 5, and 10 mg/kg/day on weeks 1, 2, and 3, respectively). In addition, two groups of mice, PBS and METH treated, were supplemented with 2% sucrose in the drinking H₂O. H₂O consumption by rodents (n = 5 per cage per group) was monitored and recorded every 7 days during 21 days of treatments. Each time point represents three independent measurements. Symbols (*, #, and ϕ) indicate higher H₂O consumption rates than in the untreated, 25 μM METH, or 2% sucrose group, respectively. Each symbol denotes P value significance (P < 0.05) calculated by ANOVA and adjusted by the use of Tukey’s multiple-comparison test. These experiments were performed twice, with similar results obtained each time.