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The effect of acute or repetitive treatments essential oil of P.nigrum (PNEO 5, 10 and 50 mg/kg, p.o.) on (A) no. of crossing (B) rearing (C) grooming in the open-field test (OFT). DZP: diazepam, 1 mg/kg (i.p.); BSP: buspirone, 10 mg/kg (i.p.). Data are represented as mean ± SEM (n = 6) and the values obtained by comparison with control group using One-way ANOVA followed by Dunnett's test, where ∗ and ∗∗ indicates p < 0.05 and p < 0.01 respectively.
