Figure 4.

Effect of PTGS1 variant on platelet aggregation, secretion, and adhesion responses. (A) Aggregation responses to arachidonic acid (AA; 1 mmol/L), adenosine diphosphate (ADP; 10 mmol/L), collagen (0.1-3 mg/mL), epinephrine (10 mmol/L), ristocetin (1.5 mg/mL), U46619 (3 mmol/L) and TRAP-6 amide (25 mmol/L) and (B) ATP secretion to AA (1 mmol/L), ADP (10 mmol/L), collagen (3 mg/mL) and TRAP-6 amide (25 mmol/L). n=20 (healthy controls; range with median); n=1 (proband); n=2 (homozygous relatives); n=4 (unaffected relatives). (C) P-selectin expression as measured by flow cytometry in whole blood stimulated by ADP (40 mmol/L), U46619 (0.5 μmol/L) or ADP plus U46619