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IL-6 and IL-8 expression in IB3-1 cells induced by SARS-CoV-2 Spike protein. (A) Flow-chart of the experimental plan. (B-E) Effects of 24 h exposure of IB3-1 cells to increasing amounts of SARS-CoV-2 spike protein (S-protein) on IL-6 (B) and IL-8 (C) mRNA and on IL-6 (D) and IL-8 (E) protein release. (F) Summary of 5 independent experiments performed using 5 nM S-protein to induce IL-6 and IL-8 increased release by treated IB3-1 cells. Results represent the average ± SEM.
