Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

[Preprint]. 2021 Apr 29:2021.04.28.441880. [Version 1] doi: 10.1101/2021.04.28.441880

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License, which allows reusers to copy and distribute the material in any medium or format in unadapted form only, and only so long as attribution is given to the creator. The license allows for commercial use.

PMC Copyright notice

Figure 1. — (A) A distinct population of Nuc_322–331-specific CD8+ T cells is detected by FACS tetramer staining in convalescent donor PID4103. Top: PBMCs from uninfected individuals were analyzed by FACS for binding to the HLA-B*40:01/Nuc_322–331 tetramer. Results are representative of 6 independent uninfected donors. Bottom: PBMCs from convalescent COVID-19 individuals from the CHIRP cohort were analyzed by FACS for binding to the HLA-B*40:01/Nuc_322–331 tetramer. Participant PID4103, but not participants PID4134 and PID 4135, harbors cells binding to the tetramer. Numbers correspond to the percentage of cells within the gates. Results are gated on live, singlet CD3+CD8+ cells.

(B) Timeline of clinical course of PID4103’s SARS-CoV-2 infection and sampling. Red indicates the dates of specific symptom initiation and resolution, blue the dates and results of SARS-CoV-2 PCR tests, and green the dates of blood draws.

(C) A distinct population of Nuc_322–331-specific CD8+ T cells is detected by CyTOF in PID4103 through dual tetramer staining. PBMCs from one uninfected individual and from PID4103 were stained with two sets of HLA-B*40:01/Nuc_322–331 tetramers conjugated to different metal lanthalides, facilitating specific detection of Nuc_322–331-specific CD8+ T cells. Numbers correspond to the percentage of cells within the gates. Results are gated on live, singlet CD3+CD8+ cells.

(D) Nuc_322–331 specific CD8+ T cells can be stimulated by the Nuc_322–331 peptide. PBMCs from PID4103 were phenotyped by CyTOF at baseline, or following 4 hours of co-stimulation with αCD49d/CD28 antibody in the absence or presence of the Nuc_322–331 peptide. Stimulations were conducted in the presence of brefeldin A to enable the detection of IFNγ. Numbers correspond to the percentage of cells within the gates. Results are gated on live, singlet CD3+CD8+ cells.