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. 2021 Apr 21;21(6):662. doi: 10.3892/etm.2021.10094

Influence of fine particulate matter and its pure particulate fractions on pulmonary immune cells and cytokines in mice

Zhouguang Jiao 1, Zhanbo Wen 1, Wenhui Yang 1, Lingfei Hu 1,✉, Jinsong Li 1
PMCID: PMC8097186  PMID: 33968192

Abstract

Particulate matter with a diameter ≤2.5 µm (PM2.5) has a complex composition and has been associated with the incidence of cardiopulmonary disease and premature death in humans. However, whether pure particulate fractions of PM2.5 (PPP2.5), which are composed primarily of carbon, are responsible for the toxicity caused by ambient particulate matter (original PM2.5 particles, OPP2.5) is currently unclear. The present study assessed the acute toxic effects of OPP2.5 sampled in Beijing, China and of its PPP2.5 fraction in male BALB/c mice. The mice were intratracheally instilled with a single dose of aerosolized OPP2.5 or PPP2.5. Blood, lungs and bronchoalveolar lavage fluid were collected after 24 h for histopathology, flow cytometry and the measurement of pro-inflammatory cytokines/chemokines and other biochemical factors. Both OPP2.5 and PPP2.5 caused acute toxicity, particularly inflammatory responses, including an increase in the levels of pro-inflammatory cytokines and an accumulation of numerous immune cells in the lungs. OPP2.5 induced a stronger inflammatory response than PPP2.5. The complex components adsorbed into the solid core granules of OPP2.5 and the granules themselves contributed to the toxic effects.

Keywords: particulate matter, acute toxicity, histopathology, immune system, inflammation

Introduction

Atmospheric particulate matter with a diameter ≤2.5 µm (PM2.5) has a large surface area, adsorbs toxic substances and penetrates into the lung alveoli (1-5). Epidemiological studies have demonstrated that ambient PM2.5 pollution contributes to lower respiratory tract infections, cancer of the trachea, bronchus and lungs, ischemic heart disease, cerebrovascular disease and chronic obstructive pulmonary disease (6-8). Therefore, ambient PM2.5 pollution increases the incidence of premature death (6,7). The elderly, children and individuals with cardiopulmonary disease are particularly susceptible to the effects of PM2.5 (6-9).

Certain toxic substances, such as some heavy metals, adsorbed into PM2.5 are released into the lungs and adsorb into lung epithelial cells, resulting in penetration to blood vessels during breathing (10-14). Therefore, the respiratory and cardiovascular systems are considered to be the primary biological targets of inhaled PM2.5 (10-14). The composition of PM2.5 is complex and varies by source (2), although it is mainly comprised of soluble salts, metals, organic compounds, carbon and biological constituents (15-17). Organic and inorganic compounds adsorbed into PM2.5 have been demonstrated to be closely associated with the biologically toxic effects of the particles (18-20). While the toxic effects of the water- and fat-soluble components of PM2.5 have been investigated (21-24), little research has been conducted on pure PM2.5 particles (PPP2.5), which are primarily comprised of carbon. Particles of carbon black have been studied (25,26); however, its toxic effects vary from those of PPP2.5. It is therefore necessary to characterize the biologically toxic effects of PPP2.5.

In previous studies (17,27), atmospheric PM2.5 was sampled from an urban area of Beijing, China, which tested the original PM2.5 particles (OPP2.5): PPP2.5 (mainly comprised of insoluble solid components), water-soluble fractions of PM2.5 and fat-soluble fractions of PM2.5. The chemical and biological constituents of these four matrices were characterized and their toxic effects on A549 human alveolar basal epithelial cells were assessed. OPP2.5 had more complex constituents than PPP2.5, including large quantities of water-soluble ions, multiple metals and polycyclic aromatic hydrocarbons, as well as copies of bacterial and fungal genomes and endotoxins (0.433 EU/mg). In contrast, PPP2.5 contained low levels of water-soluble ions and metals, practically undetectable polycyclic aromatic hydrocarbons, no bacterial or fungal genomes and endotoxin contents were <1/10 of those of OPP2.5 (0.0419 EU/mg). The main constituents of the water-soluble fractions of PM2.5 were ions, while relatively more polycyclic aromatic hydrocarbons were measured in fat-soluble fractions of PM2.5. OPP2.5 and PPP2.5 were more cytotoxic than the water-soluble and fat-soluble fractions of PM2.5. Therefore, the present study elected to further evaluate the toxic effects induced by these two insoluble solid particles in vivo.

The present study investigated the effects of OPP2.5 and PPP2.5 in male BALB/c mice treated with instilled aerosols of OPP2.5 or PPP2.5. Pro-inflammatory cytokines and chemokines and other biochemical factors were measured in serum. The lungs, the major target organs, were analyzed histopathologically. Flow cytometry was utilized to analyze immune status by counting immune cells in the lungs and the levels of inflammatory cytokines and other biochemical factors in bronchoalveolar lavage fluid (BALF) were measured. The findings provided a foundation for future research into the mechanisms of toxicity induced by inhaled PM2.5.

Materials and methods

Animals

Specific pathogen-free male BALB/c mice (n=36; 6-8 weeks old; weight, 23-29 g pre-treatment and 20-27 g post-treatment) were obtained from the Laboratory Animal Center of the Academy of Military and Medical Science. The mice were housed at 20±1˚C, with 60% humidity and 12/12 h light/dark cycles, 4 mice per cage, according to the Guideline for Animal Experiments of the Academy of Military and Medical Sciences. All animals had free access to water and food and were allowed to acclimatize for 1 week prior to the experimental procedures. All animal experiments were approved by the Animal Ethics Committee of the Academy of Military and Medical Sciences (approval no. AMMS-13-2015-013). No animals died unexpectedly prior to the end of this experiment. At the end of the experiment, the mice were euthanized via intraperitoneal injection of sodium pentobarbital (~150 mg/kg). Death was determined by cessation of heartbeat or respiratory arrest.

PM sources and processing

PM2.5 sampling and OPP2.5 and PPP2.5 processing were described previously (17). Briefly, PM was collected during May and June with a PM10/PM2.5 high-volume air sampler (Thermo Fisher Scientific, Inc.) equipped with a PM2.5 pre-separator. Collection was performed in a typical urban area in Beijing, ~18 m above ground level. PM2.5 was collected in a quartz filter (Pall Corporation) that was previously calcinated in a muffle furnace at 600˚C for 2 h to remove organic substances. OPP2.5 was prepared by resuspending with double-distilled water post-sampled filters and mixed before use. PPP2.5 was obtained by removing water- and fat-soluble components using high-temperature and high-pressure sterilization, including baking at 180˚C for 3 h to destroy microorganisms and remove endotoxins.

To prepare particle suspensions for the animal experiments, fractioned OPP2.5 and PPP2.5 particles were immediately suspended in sterile saline (0.9% NaCl) prior to use and sonicated for 10 min in ice water mixture to obtain a uniform dispersion and prevent particle aggregation.

Experimental design and execution

OPP2.5 and PPP2.5 were suspended and sonicated in sterile saline to final concentrations of 1.6 mg/ml. Mice were randomly divided into 3 groups (n=12/group) and treated with 50 µl sterile saline (control), OPP2.5 suspension or PPP2.5 suspension. According to a previous study (28), the instillation dose in the present study was ~4 times the inhaled dose of mice compared with the current atmospheric dose at 1 mg/m3 of PM2.5. Mice were weighed before and after treatment. Blood, tissues and BALF were collected 24 h post-treatment. Whole blood was collected for the determination of hematocrit and preparation of serum.

Prior to the experiment, mice were anesthetized with pentobarbital sodium (~0.08 g/kg) by intraperitoneal injection. Once a deep stage of anesthesia was attained (calm and regular breathing), mice were intratracheally instilled using a MicroSprayer Aerosolizer system (IA-1C and FMJ-250 High-Pressure Syringe; Penn-Century, Inc.) with 50 µl isotonic sterile saline solution containing 80 µg OPP2.5 or PPP2.5. Saline solution was used for the controls. Each mouse was placed in a supine position, the mouth was opened and the tongue gently moved aside using forceps to better cannulate the trachea. The particles were suspended in the appropriate solution immediately prior to instillation as aforementioned. Following instillation, the mice were immediately observed for at least 1 h before being returned to their cages under the aforementioned controlled environmental conditions.

Serum preparation and biochemical analyses

At 24 h post-treatment, ~300 µl blood was collected from one of the retro orbital sinus into a 1.5 ml centrifuge tube. Some blood was used for the determination of hematocrit. After incubating the blood at room temperature for 1-2 h, tubes were centrifuged at 825 x g for 10 min at 4˚C. The serum supernatants were collected and divided into aliquots and stored at -80˚C for the subsequent biochemical analyses on the pro-inflammatory cytokines and chemokines, and other clinical indicators.

Clinical indicators, including the activities of alanine aminotransferase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH) and alkaline phosphatase (ALP), and the levels of total protein, albumin (ALB), globulin and creatinine were measured in serum using an automatic biochemical analyzer (7080; Hitachi, Ltd.). The concentrations of pro-inflammatory cytokines/chemokines, including mouse eotaxin, interleukin (IL)1α, IL-1β, IL-6, leukemia inhibitory factor (LIF), IL-17, monocyte chemoattractant protein (MCP) 1, macrophage inflammatory protein (MIP)1α, MIP-2, vascular endothelial growth factor (VEGF) and tumor necrosis factor (TNF)α were determined in serum using Milliplex MAP multiplex immunoassay kits (Mouse Cytokine/Chemokine Panel I; MCYTOMAG-70; MilliporeSigma), according to the manufacturer's protocol. In addition, the concentration of C-reactive protein (CRP) in serum was determined using the Mouse CRP ELISA kit (cat. no. EK0977; Boster Biological Technology), according to the manufacturer's protocol. Results were normalized to the levels of hematocrit in blood. All experiments were performed in octuplicate.

Histopathology

The left lung of each mouse was submerged in 10% formalin at room temperature (RT) for 48 h and processed for histology. Paraffin blocks were prepared from dehydrated tissues (treated with gradient ethanol), and 3-µm-thick sections were stained with hematoxylin and eosin at RT for ~5 min, followed by analysis using a light microscope at x40 magnification, and the levels of inflammatory response were evaluated by assessing the alveolar structure and alveolar wall thickness in mice. All experiments were performed in triplicate.

Flow cytometry

Lung single-cell suspensions were prepared at 24 h post-treatment as described previously (29). Briefly, mice were euthanized via intraperitoneal injection of pentobarbital sodium (~150 mg/kg) following treatment with 100 µl of 500 U/ml heparin (Beijing Solarbio Science & Technology Co., Ltd.) subcutaneously. The thorax and abdomen were opened, the left atrium excised and the lungs were perfused through the right ventricle with 15 ml of phosphate-buffered saline. The trachea was exposed and cannulated, and the lung was inflated with 1 ml of digestion buffer, containing 1.5 mg/ml collagenase A (cat. no. 10103586001; Roche Diagnostics), 1.5 U/ml of dispase II protease (cat. no. D4693; Sigma-Aldrich; Merck KGaA), 0.4 mg/ml DNase I (cat. no. D8071; Sangon Biotech Co., Ltd.), 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) and 10 mM HEPES in Hanks' balanced salt solution (cat. no. 14025-092; Gibco; Thermo Fisher Scientific, Inc.). After the trachea was sealed with 3.0 sutures and the heart and mediastinal tissues were removed, the lung inflated with digestion buffer was placed in 5 ml of digestion buffer and incubated at 37˚C for 60 min with shaking. Following digestion, ~25 ml phosphate-buffered saline, 0.5% bovine serum ALB and 20 mM EDTA were added. The cell suspensions and lung tissue were filtered through a 70-µm strainer. After centrifuging the cell suspensions at 350 g at 4˚C for 5 min, the pellet was treated with red blood cell lysis solution (Tianjin Haoyang Biological Manufacture Co., Ltd.) and washed with phosphate-buffered saline in 0.5% bovine serum albumin (Amresco, LLC), then suspended in phosphate-buffered saline with 2% FBS.

After counting the lung cells using a Countess II FL automated counter (Thermo Fisher Scientific, Inc.), ~2x106 cells from each mouse were incubated in blocking solution, consisting of phosphate-buffered saline containing 0.25% FcBlock (cat. no. 553141; BD Biosciences), for 15 min at 4˚C and then stained with antibodies (a list of antibodies, clones, fluorochromes and concentrations are presented in Table SI) for 30 min at 4˚C. Following staining, cells were washed and fixed with 1% paraformaldehyde in phosphate-buffered saline for 30 min at 4˚C, then washed and resuspended in phosphate-buffered saline with 0.5% bovine serum albumin. Flow cytometry was conducted with a BD FACSymphony A5 flow cytometer using BD FACSDiva software 8.0 (BD Biosciences), and the data were analyzed using FlowJo software (version 10; FlowJo LLC). The present study used a previously published protocol (30) to analyze the immune status in the lung. Live CD45+ cells were gated, and myeloid cells were gated based on CD11c vs. CD11b. Total myeloid cells were then plotted as MerTK vs. CD64, and the MerTK+ CD64+ macrophage gate was plotted as CD11c vs. MHC II to illustrate the alveolar macrophages and interstitial macrophage (IM)1, IM2 and IM3. A macrophage-deficient gate was plotted with CD11c and MHC II to illustrate dendritic cells, which were plotted as CD11c vs. CD11b to identify Batf3+ and Irf4+ dendritic cells. A macrophage/dendritic cell-deficient gate was plotted as Ly6G vs. CD11b to identify neutrophils (CD11b+ Ly6G+) and a macrophage/dendritic cell/neutrophil-deficient gate was plotted as side scatter vs. F4/80 to identify eosinophils and monocytes. All experiments were performed in quadruplicate.

BALF collection and biochemical analyses

Following sacrifice 24 h post-treatment, the tracheas were exposed and cannulated. The lungs were lavaged twice, each time by instilling a 0.7 ml aliquot of isotonic saline solution and the two washes were pooled as BALF. The total lavage fluid from each mouse lung was combined and centrifuged at 400 x g for 10 min at 4˚C (3-18K; Sigma-Aldrich; Merck KGaA) and the supernatants were divided into aliquots and stored for subsequent determination. Levels of clinical indicators and pro-inflammatory cytokines/chemokines were measured in BALF as aforementioned for serum analyses. All experiments were performed in octuplicate.

Statistical analysis

SAS software (version 9.3; SAS Institute, Inc.) was used to analyze data. With the exception of the histopathological evaluation, all data are expressed as mean ± standard error of the mean. Statistical differences were assessed by one-way ANOVA followed by Student-Newman-Keuls comparisons. Non-parametric Kruskal-Wallis test was performed followed by Dunn's test for data that did not conform to normality or homogeneity of variance. P<0.05 was considered to indicate a statistically significant difference.

Results

Body weight

Mice were weighed before and after treatment with OPP2.5 and PPP2.5 (Fig. 1). Body weight decreased in the treated groups compared with controls. There was no significant difference between mice treated with OPP2.5 and PPP2.5.

Figure 1.

Figure 1

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Changes in mouse body weight following intratracheal instillation with aerosolized OPP2.5 particles (80 µg), PPP2.5 particles (80 µg) or vehicle (50 µl sterile saline). Data are presented as the mean ± standard error of the mean (n=8/group). **P<0.01 vs. control. OPP2.5, original atmospheric particulate matter with a diameter ≤2.5 µm; PPP2.5, pure atmospheric particulate matter with a diameter ≤2.5 µm.

Serum biochemical analyses

The activities or contents of AST, globulin, total protein and creatinine in serum did not differ significantly between the treatment and control groups (data not shown). Compared with levels in the control group, the blood hematocrit in the OPP2.5 group was significantly higher compared with controls (Fig. 2A). Mice treated with OPP2.5 or PPP2.5 had lower serum ALT, LDH and ALP activities compared with controls (Fig. 2B, D and E). Furthermore, serum ALB levels decreased in the OPP2.5 group compared with controls (Fig. 2C). With the exception of ALB and hematocrit, clinical indicator levels did not differ significantly between the OPP2.5 and PPP2.5 groups.

Figure 2.

Figure 2

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Levels of biochemical factors in blood and serum following exposure to OPP2.5 particles (80 µg), PPP2.5 particles (80 µg) or vehicle (50 µl sterile saline). (A) Hematocrit level. Relative activities of (B) ALT, (C) ALB, (D) LDH and (E) ALP. (F) Relative content of MCP1. Results were normalized to hematocrit levels. Data are presented as mean ± standard error of the mean (n=8/group). *P<0.05 and **P<0.01 vs. control. #P<0.05 vs. OPP2.5 group. OPP2.5, original atmospheric particulate matter with a diameter ≤2.5 µm; PPP2.5, pure atmospheric particulate matter with a diameter ≤2.5 µm; ALT, alanine aminotransferase; ALB, albumin; LDH, lactate dehydrogenase; ALP, alkaline phosphatase; MCP1, monocyte chemoattractant protein.

Serum levels of eotaxin, IL-1α, IL-1β, IL-6, LIF, IL-17, MIP-1α, MIP-2, VEGF, TNF-α and CRP did not differ significantly between groups (data not shown). Serum MCP-1 levels increased in the OPP2.5 group compared with the control and PPP2.5 groups (Fig. 2F).

Histopathology

Lung tissue structure in the control group was normal, with almost all alveolar walls intact. There were interstitial inflammatory changes in the lungs of mice treated with OPP2.5 or PPP2.5 (Fig. 3). These included a widening of the alveolar septa and inflammatory cell infiltration. Inflammatory responses did not differ between the OPP2.5 and PPP2.5 groups.

Figure 3.

Figure 3

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Stained sections of lung tissues following exposure to OPP2.5 particles (80 µg), PPP2.5 particles (80 µg) or vehicle (50 µl sterile saline). Widening alveolar septa (black arrows) and notable inflammatory cell infiltration were induced by OPP2.5 and PPP2.5. Magnification, x40 (n=3/group). OPP2.5, original atmospheric particulate matter with a diameter ≤2.5 µm; PPP2.5, pure atmospheric particulate matter with a diameter ≤2.5 µm.

Immune status

The flow cytometry gating scheme of the present study is shown in Fig. 4A. The number of CD45+ cells in the lungs from the treated groups was higher compared with that from the control group (Fig. 4B). Among these, the numbers of interstitial macrophages (Fig. 4D) and neutrophils (Fig. 4E) increased significantly in the treated groups compared with in the control group. Additionally, the numbers of alveolar macrophages (Fig. 4C), dendritic cells (Fig. 4F) and monocytes (Fig. 4I) increased significantly in the OPP2.5 group but not in the PPP2.5 group compared with in the control group. The most accumulated dendritic cells in the OPP2.5 group were Irf4+ cells (Fig. 4H), not Batf3+ cells (Fig. 4G). The numbers of IM2 and 3 cells and eosinophils did not differ between the treated and control groups (Fig. S1).

Figure 4.

Figure 4

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Comparison of pulmonary immune cell numbers obtained by flow cytometry following exposure to OPP2.5 particles (80 µg), PPP2.5 particles (80 µg) or vehicle (50 µl sterile saline). (A) Fluorescence-activated cell sorting gating strategy used to identify pulmonary major immune cells. Live CD45+ cells were gated and myeloid cells were gated on CD11c vs. CD11b. Total myeloid cells were then plotted as MerTK vs. CD64 and the MerTK+ CD64+ macrophage gate was plotted as CD11c vs. MHC II to illustrate the AMs and IM1, IM2 and IM3. A macrophage-deficient gate was plotted with CD11c and MHC II to illustrate DCs, which were plotted as CD11c vs. CD11b to identify Batf3+ and Irf4+ DCs. A macrophage/DC-deficient gate was plotted as Ly6G vs. CD11b to identify neutrophils (CD11b+ Ly6G+) and a macrophage/DC/neutrophil-deficient gate was plotted as SSC vs. F4/80 to identify eosinophils and monocytes. (B) The proportion of CD45+ cells in living cells and the absolute number of CD45+ cells in each sample from treated and control groups. The proportion of (C) AMs, (D) IM1s, (E) neutrophils, (F) DCs, (G) Batf3+, (H) Irf4+ and (I) monocytes in CD45+ cells and their absolute number. The circles represent the corresponding data of each mouse in the control group, the squares represent the corresponding data of each mouse in the OPP2.5 group, and the triangles represent the corresponding data of each mouse in the PPP2.5 group. Data are presented as mean ± standard error of the mean (n=4/group). *P<0.05 and **P<0.01 vs. control. #P<0.05 and ##P<0.01 vs. OPP2.5 group. OPP2.5, original atmospheric particulate matter with a diameter ≤2.5 µm; PPP2.5, pure atmospheric particulate matter with a diameter ≤2.5 µm; CD, cluster of differentiation; MHC, major histocompatibility complex; AMs, alveolar macrophages; IM, interstitial macrophage; DC, dendritic cell; SSC, side scatter.

BALF biochemical analyses

BALF levels of ALT, ALB, creatinine, globulin, total protein and ALP did not differ significantly between the treated and control groups (data not shown). The activity of AST (Fig. 5A) and LDH (Fig. 5B) were significantly higher in BALF from the treated groups compared with controls; however, there was no significant difference between the OPP2.5 and PPP2.5 groups. These results indicated that acute inhalation of OPP2.5 or PPP2.5 damages alveolar-capillary barriers and cell membranes in the lung.

Figure 5.

Figure 5

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Levels of biochemical factors (A) AST, (B) LDH, (C) eotaxin, (D) MCP-1, (E) MIP-1α, (F) MIP-2, (G) IL-1β, (H) TNF- α, (I) IL-6, (J) IL-17, (K) LIF, (L) VEGF and (M) CRP in BALF from mice treated with OPP2.5 particles (80 µg), PPP2.5 particles (80 µg) or vehicle (50 µl sterile saline). Data are presented as mean ± standard error of mean (n=8/group). *P<0.05 and **P<0.01 vs. control. #P<0.05 and ##P<0.01 vs. OPP2.5 group. AST, aspartate transaminase; LDH, lactate dehydrogenase; MCP, monocyte chemoattractant protein; MIP, macrophage inflammatory protein; IL, interleukin, TNF, tumor necrosis factor; LIF, leukemia inhibitory factor; VEGF, vascular endothelial growth factor; CRP, C-reactive protein; BALF, bronchoalveolar lavage fluid; OPP2.5, original atmospheric particulate matter with a diameter ≤2.5 µm; PPP2.5, pure atmospheric particulate matter with a diameter ≤2.5 µm.

IL-1α levels in BALF did not differ between groups (data not shown). Levels of eotaxin, MCP-1, MIP-1α, MIP-2, IL-1β, TNF-α, IL-6, IL-17, LIF, VEGF and CRP were significantly higher in the treated groups compared with controls (Fig. 5C-M). Levels of MIP-1α and MIP-2 were higher in the PPP2.5 group compared with the OPP2.5 group. Levels of IL-1β and IL-6 were higher in the OPP2.5 group compared with the PPP2.5 group.

Discussion

OPP2.5 has more complex constituents than PPP2.5 (27). The composition of PPP2.5 is simple for relatively pure solid particles, having almost no complex constituents adsorbed into particle surfaces. The aim of the present study was to determine whether pure solid PM2.5 exerts toxic effects similar to those of original PM2.5 with its complex constituents. The results demonstrated that mouse body weight decreased to varying degrees following acute exposure, perhaps due to the influence of anesthesia. However, treatment still decreased body weight. Hematocrit levels in mice treated with OPP2.5 were slightly elevated, suggesting minor dehydration. Decreased activity of ALT, LDH and ALP in mice treated with OPP2.5 and PPP2.5 and decreased ALB in mice treated with OPP2.5 indicated that the treatment may have induced mild malnutrition, which may explain the observed weight loss.

Serum levels of cytokine MCP-1 were higher in the OPP2.5 group compared with the control and PPP2.5 groups. MCP-1 promotes monocyte adhesion to vascular endothelial cells, which enables mononuclear cells to mediate immune phagocytosis and scavenging (31). The results indicated that OPP2.5 and PPP2.5 cause acute toxic effects at the whole-body level, such as weight loss and mild malnutrition. Furthermore, OPP2.5 causes minor dehydration and systemic inflammation.

To assess lung toxicity, lung damage was evaluated using histopathology, localized counts of major immune cells and cytokines, and clinical parameters. Lung histopathology reported inflammatory changes in the treated groups, with inflammatory cell infiltration into the alveolar interstitial space. This was confirmed by flow cytometry, which recorded numerous immune cells (cluster of differentiation 45+ cells) in the lungs of treated mice, particularly IM1 and neutrophils in both treated groups and alveolar macrophages, dendritic cells and monocytes in the OPP2.5 group. Neutrophils have been proposed to mediate acute lung injury (32) and IM1 form the branch of interstitial macrophages that influence pulmonary fibrotic processes (33); however, its function in PM-treated mice requires further study. Alveolar macrophages usually inhabit the position of interface between pulmonary mucosa and the external environment, sense immunostimulation directly and maintain immune tolerance (33). Dendritic cells are important regulators in the innate and adaptive immune responses (34). The results demonstrated that PM, particularly OPP2.5, stimulate the innate immune response in mice, leading to the accumulation of numerous immune cells in the lung 24 h post-treatment.

Treatment with PM increased AST and LDH in BALF in the present study. AST was primarily derived from plasma exudation and reflects damage to the alveolar-capillary barrier. LDH, an enzyme located in the cytoplasm, is released when cell membranes are damaged or disintegrate following cell death (35). The results indicated that, to a certain degree, cell membranes and parenchymal cells in the lung were damaged by OPP2.5 and PPP2.5.

The levels of multiple pro-inflammatory cytokines/chemokines increased in BALF following treatment in the present study. Eotaxin is a chemoattractant that recruits eosinophils (36) and MIP-1α attracts lymphocytes, monocytes and neutrophils to an inflammatory infection site and mediate immune cell infiltration, positioning and activation in tissues (37). MIP-2 specifically recruits neutrophils, macrophages and lymphocytes to sites of inflammation following particle exposure (38) and enhances neutrophil participation in immune responses (16). This explains the observed inflammatory cell infiltration via histopathology and flow cytometry, which demonstrated the accumulation of macrophages and neutrophils in both treated groups and monocytes in the OPP2.5 group. Numerous eosinophils in the lung were not reported and the higher levels of MIP-1α and MIP-2 in the PPP2.5 group did not correspond to the expected accumulation of macrophages, neutrophils and monocytes. This may be due to eotaxin measured in BALF not being effectively released into the bloodstream in the PPP2.5 group or that other cytokines are involved in the chemotaxis of macrophages, neutrophils and monocytes to the lung.

IL-6 is an important mediator of fever and of acute-phase responses. It is also an anti-inflammatory factor that inhibits pulmonary inflammation and fibrosis (39). TNF-α is involved in apoptosis, cell differentiation and cell recruitment (40). As early biochemical mediators of pulmonary inflammation (41), TNF-α and IL-1 are involved in the initial stage of inflammation (42) and are released by resident macrophages to promote adhesion of circulating inflammatory cells to the endothelium (42). These two cytokines also induce the release of chemoattractant factors, including IL-6, MIP-2 and MCP-1, all of which attract inflammatory cells into the alveoli (16,41,43) and enhance inflammatory and immune responses. IL-1α, IL-1β and TNF-α increase mRNA expression of LIF (44), an anti-inflammatory factor that is upregulated during peripheral inflammation (45). VEGF can bind to the VEGF receptor on the surface of vessel endothelial cells, enhancing vascular permeability and promoting the formation of blood vessels (46,47). Upregulation of VEGF is often interpreted as a protective attempt to restore tissue homeostasis (48,49). Analyses of pro-inflammatory cytokines in BALF indicated extensive inflammation in the lungs of mice treated with OPP2.5 or PPP2.5 24 h post-treatment, with higher levels of IL-1β and IL-6 in the OPP2.5 group. These findings were consistent with the flow cytometry analysis, which recorded numerous immune cells, including neutrophils and macrophages, in the lungs of treated mice, particularly in the OPP2.5 group. However, due to precise regulatory networks, increased levels of cytokines promoting inflammatory and immune responses triggered a concomitant increase in the levels of LIF, which can limit excessive responses (50).

Overall, the results of the current study for OPP2.5 were consistent with those of previous reports, suggesting that intratracheal instillation of PM in rodents induced pulmonary and systemic inflammation (16,51-54). Previous studies have demonstrated that water-soluble components and metals such as manganese, chromium, titanium, iron, copper, zinc, nickel and molybdenum in particulate matter may be associated with the generation of reactive oxygen species (55,56). Oxidative stress is considered to be positively associated with induction of inflammation (57). In the present study, PPP2.5, which is comprised of pure solid particles without complex constituents adsorbed into the surface, caused pulmonary inflammation in mice. However, the systemic and pulmonary inflammatory responses were weaker than those induced by OPP2.5. The reason may be that less water-soluble components and metals were absorbed onto the surface of PPP2.5, as demonstrated by a previous study (27). These results provide a new perspective on the acute toxicity of PM and a reference for evaluating other pollutants. The acute toxicity of pure solid particles should be considered in tandem with that of the constituents that adsorb onto the particle surface.

Additionally, the toxicities and constituents of particles may be associated with wind direction, region or/and season (58,59). These factors were not considered in the present study; however, they have little effect on the conclusion. However, one limitation of the present study was that the normal daily inhaled dose of the mice at the beginning of the study was not considered and should be investigated by future studies. Another limitation was that the local expression level of cytokines in the lungs was not assessed, and histological score was not performed, which could be investigated in future studies. Furthermore, the small number of mice in each group for flow cytometry may also be a limitation, which can be improved in future studies, and the toxicity of chronic exposure to pure solid particles should be assessed in the future.

Supplementary Material

Pulmonary immune cell numbers counted by flow cytometry following exposure to OPP2.5 particles (80 μg), PPP2.5 particles (80 μg) or vehicle (50 μl sterile saline). Percentage and absolute number of (A) IM2, (B) IM3 and (C) eosinophil cells in CD45+ cells. The circles represent the corresponding data of each mouse in the control group, the squares represent the corresponding data of each mouse in the OPP2.5 group, and the triangles represent the corresponding data of each mouse in the PPP2.5 group. Data are presented as mean ± standard error of the mean (n=4/group). **P<0.01 vs. control. OPP2.5, original atmospheric particulate matter with a diameter ≤2.5 μm; PPP2.5, pure atmospheric particulate matter with a diameter ≤2.5 μm; IM, interstitial macrophage; CD, cluster of differentiation.
Supplementary_Data.pdf (227.9KB, pdf)
Immunophenotyping antibodies.
Supplementary_Data.pdf (227.9KB, pdf)

Acknowledgements

Not applicable.

Funding Statement

Funding: The present study was supported by the National Science Foundation of China (grant no. 41205102).

Availability of data and materials

All data generated and/or analyzed during the present study are included in this published article.

Authors' contributions

ZJ, ZW, LH and JL designed the present study. ZJ, LH and WY performed the experiments. ZJ analyzed data and wrote the manuscript draft. JL and ZW revised the final manuscript. ZW and LH confirm the authenticity of all the raw data. All authors read and approved the final manuscript.

Ethics approval and consent to participate

All animal experiments in the present study were approved by the Animal Ethics Committee of the Academy of Military and Medical Sciences (approval no. AMMS-13-2015-013).

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Pulmonary immune cell numbers counted by flow cytometry following exposure to OPP2.5 particles (80 μg), PPP2.5 particles (80 μg) or vehicle (50 μl sterile saline). Percentage and absolute number of (A) IM2, (B) IM3 and (C) eosinophil cells in CD45+ cells. The circles represent the corresponding data of each mouse in the control group, the squares represent the corresponding data of each mouse in the OPP2.5 group, and the triangles represent the corresponding data of each mouse in the PPP2.5 group. Data are presented as mean ± standard error of the mean (n=4/group). **P<0.01 vs. control. OPP2.5, original atmospheric particulate matter with a diameter ≤2.5 μm; PPP2.5, pure atmospheric particulate matter with a diameter ≤2.5 μm; IM, interstitial macrophage; CD, cluster of differentiation.
Supplementary_Data.pdf (227.9KB, pdf)
Immunophenotyping antibodies.
Supplementary_Data.pdf (227.9KB, pdf)

Data Availability Statement

All data generated and/or analyzed during the present study are included in this published article.

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