Erratum

In1, there were errors in the figure legends published on pages 1332, 1333, 1334, 1335.

The correct figure legends should read as below:

Figure 2. Characterizing bone marrow aspirate derived cultures. (A) Bone marrow mesenchymal stem cell (BM‐MSC) phenotyping using flow cytometry showing positive staining for markers of MSCs; CD105, CD90, CD73 and the absence of heme‐lineage markers (Hemato); CD14, CD19, CD34, CD45, and HLA‐DR. The bars show the mean positive percentage of BM cultured cells (n = 3) with error bars representing standard deviation. Images of tri‐lineage differentiation for adipogenesis (B), osteogenesis (C), and chondrogenesis (D), the black bars represent 500 µm.

Figure 3. Platelet product composition's impact on bone marrow mesenchymal stem cell (BM‐MSC) proliferation. XTT assay quantifying BM‐MSC proliferation following 4‐day exposure to media containing 10% platelet products or fetal calf serum (FCS) as control. Proliferation was represented as optical density normalized to FCS. (A) Cultured BM‐MSCs were exposed to 10% platelet lysate (PL), clinical standard platelet lysate (CPL), and FCS. (B) Cultured BM‐MSCs were exposed to autologous 10% PL and CPL as well as 10% FCS. (C) The change in platelets and leukocytes from whole blood to platelet‐rich product (PRP) and filtered PRP (fPRP) (compared using a paired t test). (D) Cultured BM‐MSCs were exposed to 10% PL, fPL, and FCS from three PL donors. One‐way analysis of variance test was used to test significance between the platelet products effects on proliferation (A, B, and D) (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001). Except for (B), all experiments were performed on a minimum of three different platelet product (PP) donors and three BM‐MSC cultures. Error bars indicate variation between PP except for (B) where error bars indicate technical variation between replicates.

Figure 4. Platelet product composition's impact on bone marrow mesenchymal stem cell (BM‐MSC) migration. The IncuCyte transwell assay quantifies BM‐MSCs moving toward a chemotactic gradient. (A) Representative images of wells of 0.5% fetal calf serum (FCS) (top), 10% FCS (middle), and 10% platelet lysate (PL) (bottom). Arrows indicate pores (black), static cells on the top of the transwell (purple), and cells that have migrated (blue). The processing mask that quantifies the migrated cells is shown in green. (B) Representative time‐course response of the cells from one BM‐MSC culture that have migrated through the transwell toward 10% PL, 10% clinical standard platelet lysate (CPL), 10% FCS, and 0.5% FCS. Data are shown as the area of the bottom of the well occupied by cells. (C) Average object area of the underside of the transwell occupied by BM‐MSCs and treated with 10% PL, 10% CPL, and 10% FCS was normalized to 10% FCS. (D) Representative time‐course response of the cells from one culture that have migrated through the transwell toward 10% PL, 10% fPL, 10% FCS, and 0.5% FCS. (E) Average object area of the underside of the transwell occupied by BM‐MSCs and treated with 10% PL, 10% fPL, and 10% FCS was normalized to 10% FCS. A one‐way analysis of variance was carried out using the Kruskal–Wallis test for normality (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001). Error bars indicate variation between PL donors. Experiments in (C) and (E) were performed on a minimum of three different BM‐MSC cultures.

Figure 5. Clinical standard platelet lysate (CPL)‐loaded membrane supports cell proliferation in fibroblastic‐colony‐forming unit (CFU‐F) assay. (A) Representative CFU‐F dishes of bone marrow mesenchymal stem cells (BM‐MSCs) grown in either standard expansion media (top dish) or serum‐free Dulbecco's modified Eagle's medium (DMEM) with the additional CPL‐loaded membrane as a source of released growth factors and cytokines (bottom dish). (B) Representative individual colonies of cells grown in expansion media (top) or CPL‐loaded membrane and DMEM (bottom). Three BMA donors were tested on a single CPL product. Images were collected using a photo scanner at 1,200 dpi. (C) Comparison of average colony area, density, and total number between cells treated with a CPL‐loaded scaffold and expansion media (EM). An unpaired t test found no significant difference between CPL of EM.

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