Abstract
Twenty-one heads of cattle were vaccinated with Madibovin, 31 with Rabdomun and 127 with Rabisin on 4 different farms. Rabies neutralizing antibody titre (≥0.5 IU/ml) was detected in 80% of 163 animals tested about 1 month and in 42% of 133 animals tested about 1 year after primary vaccination. On 3 of the farms 86 animals received booster vaccination about 1 year after primary vaccination. All these animals had antibody titre (≥0.5 IU/ml) about 1 month after booster and antibody levels were higher than after the primary vaccination. Rabies antibody titres (≥0.5 IU/ml) were detected in 96% of 50 animals tested 1 year after the booster. No significant differences (p>0.05) in antibody levels were detected between animals vaccinated with Madibovin or Rabisin (farm C) respectively with Rabisin or Rabdomun (farm D) at any collection time. Responses to rabies vaccines varied considerably between the farms. After primary vaccination of the animals on 2 farms with the same batch of Rabisin, the antibody levels clearly differed (p<0.0001) between the farms.
Our results indicate that booster is always necessary after primary vaccination to ensure that all animals are protected.
Keywords: field conditions, Finland
Sammanfattning
Tjugoett nötkreatur vaccinerades med Madibovin, 31 med Rabdomun och 127 med Rabisin mot rabies på 4 besättningar. Antikropps-titer (≥0.5 IU/ml) kunde påvisas hos 80% av 163 testade djur en månad och hos 42% av 133 testade djur 1 år efter vaccinationen. Boostervaccination utfördes på 86 kvarvarande djur i 3 besättningar 1 år efter primärvaccinationen. Alla djur hade antikropps-titer (>0.5 IU/ml) en månad efter boostern och titren var högre än efter primärvaccinationen. Antikropps-titer påvisades hos 96% av 50 testade djur 1 år efter boostern. Inom besättningarna kunde ingen signifikant skillnad i titer (p>0.05) påvisas mellan djur som vaccinerats med Rabisin eller Madibovin (farm C) respektive med Rabdomun eller Rabisin (farm D). Immunreaktionen varierade mellan besättningarna. Signifikant skillnad i titer (p<0.0001) kunde påvisas efter primärvaccinationen mellan djuren i 2 besättningar vaccinerade med samma batch Rabisin.
Resultaten visar, att booster alltid är nödvändig efter primär- vaccination, för att alla djur skall bli skyddade.
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Acknowledgments
We thank veterinary surgeons Terttu Katila, Tiina Kotilainen, Laura Kulkas, Ritva Tynkkynen and Seppo Ukkola for vaccinating the cattle and taking blood samples. The work was supported in part by the Ministry of Agriculture and Forestrythe Veterinary and Food Department, Helsinki, Finland.
References
- Anonymous: WHO Expert Committee on Rabies. Seventh Report, WHO Technical Report Series 709, WHO, Geneva, 1984. [PubMed]
- Nyberg M, Kulonen K, Neuvonen E, Ek-Kommonen C, Nuorgam M, Westerling B. An epidemic of sylvatic rabies in Finland - Descriptive epidemiology and results of oral vaccination. Acta vet. scand. 1992;33:43–57. doi: 10.1186/BF03546935. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sihvonen L, Kulonen K, Soveri T, Nieminen M. Rabies antibody titres in vaccinated reindeer. Acta vet. scand. 1993;34:199–202. doi: 10.1186/BF03548210. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith JS, Yaeger PA, Baer GM. A rapid reproducible test for determing rabies neutralizing antibody. Bull. WHO. 1973;48:535–541. [PMC free article] [PubMed] [Google Scholar]
- Soulebot JP, Precausta P, Brun A, Blancou JM, Pepin M, Chappuis G, Petermann HG. Immunisation of herbivores against rabies using an inactivated cell culture vaccine. In: Kuwert E, Merieux C, Koprowski H, Bögel K, editors. Rabies in the tropics. 1985. pp. 274–284. [Google Scholar]
- Turner GS. Humoral and cellural immune responses of mice to rabies and smallpox vaccines. Nature New Biol. 1973;247:90–92. doi: 10.1038/newbio241090a0. [DOI] [PubMed] [Google Scholar]
- Turner GS. Immunoglobulin (IgG) and (IgM) antibody response to rabies vaccine. J. gen. Virol. 1978;40:595–604. doi: 10.1099/0022-1317-40-3-595. [DOI] [PubMed] [Google Scholar]
- Wiktor TJ, MacFarlan RI, Foggin CM, Koprowski H. Antigenic analysis of rabies and Mo- kola virus from Zimbabwe using monoclonal antibodies. Dev. Biol. Stand. 1984;57:199–211. [PubMed] [Google Scholar]