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. 2021 May 6;253(6):117. doi: 10.1007/s00425-021-03634-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Subcellular localization of ScZRC1 in plant. ScZRC1::eGFP fusion protein (upper panel) and eGFP alone (cytoplasmic control, lower panel) were transiently expressed in A. thaliana leaf protoplasts by PEG-mediated transfection, and analyzed using fluorescence microscopy. BF, picture taken upon illumination in bright field; eGFP, fluorescence of the fusion protein ScZRC1::eGFP; Auto, chloroplasts revealed by chlorophyll autofluorescence; Merged, the two signals for eGFP and Auto were merged in the same image. Scale bar = 10 µm