Figure 1.

Absence of Batf3 leads to enrichment of lineage-intermediate cDCs

(A and B) MHCII+ CD11c+ splenic cDCs of C57BL/6 WT and Batf3−/− mice were assessed for expression of CD8 and CD11b by imaging flow cytometry. (A) Pre-gated to MHCII+ CD11c+ cells, cDC1 cells were identified as CD8+CD11b−, cDC2 cells as CD8−CD11b+, and lineage-intermediate cDCs as CD8+CD11b+ (Figure S1). BF, brightfield; merge, overlay of CD8 and CD11b. (B) Intensities of CD8 and CD11b was compared between C57BL/6 WT and Batf3−/− cDC lineages. Each data point represents an individual cell with mean and interquartile range indicated. (C) cDCs in splenocytes of C57BL/6 WT and Batf3-/- mice (n=5) were immunoprofiled using conventional flow cytometry. The number of CD8+CD11b+ cDCs per 100,000 B cells was compared. Statistical significance was determined using one-way ANOVA followed by Tukey's multiple comparison test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Shown is one of two independent experiments. See also Figure S1.