Fig. 3.

Distinct effects of Rap and Rap + Met treatments on liver proteome remodeling. Frequency distribution of individual liver protein synthesis rates relative to control for male (A) and female (B) mice. Effects of Rap and Rap + Met treatments on log₂-fold change (FC) from control in protein content (C and F), fractional protein synthesis (D and G), and absolute protein synthesis (E and H) in specific cellular compartments from male (C, D, and E, respectively) and female (F, G, and H, respectively) liver cytosolic-/mitochondrial-enriched proteome. Also, plots of Rap and Rap + Met treatments on log₂-fold change (FC) from control in fractional protein synthesis rates (I and K) or absolute synthesis rates (J and L) by the log₂-fold change (FC) from control in abundance of specific cellular compartments of the liver proteome in males (I and J) and females (K and L). b = significantly different than Rap, P < 0.05