Skip to main content
NCBI home page
Acta Veterinaria Scandinavica logoLink to Acta Veterinaria Scandinavica
. 1993 Jun 1;34(2):109–116. doi: 10.1186/BF03548198

Intraluminal Pressure Variations in the Isthmus of the Porcine Oviduct after Intrauterine Insemination with Saline, Oestrogen Solution or Boar Seminal Plasma

A Pettersson 1,✉, S Einarsson 1, H Kindahl 1
PMCID: PMC8112501  PMID: 8266887

Abstract

The purpose of this study was to investigate if an intrauterine deposition of saline, boar seminal plasma, or an oestrogen solution containing 11.5 µg oestrogens affected the intraluminal pressure in the isthmus of the oviduct in unrestrained gilts. In order to monitor variations in intraluminal pressure, 2 ultra-miniature pressure sensors, located at 2 different points along the same isthmus were used. After an initial recording period, either saline, boar seminal plasma, or the oestrogen solution was deposited directly into the uterus. Intraluminal pressure recordings were conducted up to 6 h after insemination, and blood samples, for monitoring oestradiol-17B and 15-ketodihydroprostaglandin F2α levels, were collected. None of the inseminates had any consistent effect on the intraluminal pressure in the porcine oviduct. After deposition of the oestrogen solution, increases in the circulating levels of both oestradiol-17B and 15-ketodihydropro- staglandin F2α were seen.

Keywords: prostaglandin F2α

Full Text

The Full Text of this article is available as a PDF (1.0 MB).

Acknowledgments

This work has been supported by the Swedish Council for Forestry and Agricultural Research. Special thanks to Birgitta Berner and Marie Sundberg for secretarial help and to the Department of Clinical Chemistry for performing the steroid analysis.

References

  1. Boilert B, Edqvist L-E, Johansson EDB, Lindberg P, Martinsson K. The influence of conjugated estrogens in radioimmunoassay using different antibodies against estradiol-17ß. Steroids. 1973;22:891–894. doi: 10.1016/0039-128X(73)90060-3. [DOI] [PubMed] [Google Scholar]
  2. Claus R, Ellendorff F, Hoang-Vu C. Spontaneous electromyographic activity throughout the cycle in the sow and its change by intrauterine oestrogen infusion during oestrus. J. Reprod. Fert. 1989;89:543–551. doi: 10.1530/jrf.0.0870543. [DOI] [PubMed] [Google Scholar]
  3. Claus R, Meyer H-D, Giménez T, Hoang-Vu C, Münster E. Effect of seminal oestrogens of the boar on prostaglandin F2a release from the uterus of the sow. Anim. Reprod. Sei. 1990;23:145–156. doi: 10.1016/0378-4320(90)90056-L. [DOI] [Google Scholar]
  4. Claus R, Schopper D, Hoang-Vu C. Contribution of individual compartments of the genital tract to oestrogen and testosterone concentrations in ejaculates of the boar. Acta endocr. (Copenh.) 1985;109:281–288. doi: 10.1530/acta.0.1090281. [DOI] [PubMed] [Google Scholar]
  5. Granström E, Kindahi H. Radioimmunoassay of the major plasma metabolite of PGF2a, 15-keto-13, 14- dihydro-PGF2a. Meth. Enzymol. 1982;86:302–339. doi: 10.1016/0076-6879(82)86204-6. [DOI] [PubMed] [Google Scholar]
  6. Ham EA, Cirillo VJ, Zanetti ME, Kuehl FA. Estro-gen-directed synthesis of specific prostaglandins in uterus. Proc. Nat. Acad. Sei. USA. 1975;72:1420–1424. doi: 10.1073/pnas.72.4.1420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Henriksson A, Gustavsson A, Einarsson S. A new method for continuous recording of oviductal pressure variations in unrestrained gilts. Acta physiol, scand. 1987;131:303–307. doi: 10.1111/j.1748-1716.1987.tb08241.x. [DOI] [PubMed] [Google Scholar]
  8. Hoang-Vu C. Thesis Sei. Agr. 1987. Elektrische Aktivität des Myometriums beim Schwein während des Zyclus und ihre Beeinflussung durch Infusion von Östrogenen in das Uteruslumen am Tag der Rausche. (Uterine electromyographic activity of the sow during the sexual cycle and after intrauterine application of seminal oestrogens at standing oestrus) [Google Scholar]
  9. Kindahi H, Knudsen O, Madej A, Edqvist L-E. Progesterone, prostaglandin F-2(X, PMSG and oestrone sulphate during early pregnancy in the mare. J. Reprod. Fert. Suppl. 1982;32:353–359. [PubMed] [Google Scholar]
  10. Kunavongkrit A, Kindahi H, Madej A. Clinical and endocrinological studies in primiparous zero-weaned sows: 2. Hormonal patterns of normal cycling sows after zero-weaning. Zbl Vet.-Med. A. 1983;30:616–624. doi: 10.1111/j.1439-0442.1983.tb01024.x. [DOI] [PubMed] [Google Scholar]
  11. Pettersson A. Cyclic variations in intraluminal pressure in the isthmus of the oviduct in unrestrained gilts. J. Vet. Med. A. 1991;38:337–343. doi: 10.1111/j.1439-0442.1991.tb01019.x. [DOI] [PubMed] [Google Scholar]
  12. Rodriguez-Martinez H, Einarsson S. Influence of prostaglandins on the spontaneous motility of pig oviducts. Anim. Reprod. Sei. 1985;8:259–279. doi: 10.1016/0378-4320(85)90031-4. [DOI] [Google Scholar]
  13. Rodriguez-Martinez H, Kunavongkrit A. Chronical venous catheterization for frequent blood sampling in unrestrained pigs. Acta vet. scand. 1983;24:318–320. doi: 10.1186/BF03546736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Viring S, Einarsson S. Effect of boar seminal plasma on uterine and oviductal motility in oestrous gilts. Acta vet. scand. 1980;21:607–616. doi: 10.1186/BF03546848. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Viring S, Einarsson S, Jones B, Larsson K. Transuterine transport of small- and medium-sized molecules deposited in the uterus in gilts. J. Reprod. Fert. 1980;59:459–462. doi: 10.1530/jrf.0.0590459. [DOI] [PubMed] [Google Scholar]

Articles from Acta Veterinaria Scandinavica are provided here courtesy of BMC

RESOURCES