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Acta Veterinaria Scandinavica logoLink to Acta Veterinaria Scandinavica
. 1993 Sep 1;34(3):231–236. doi: 10.1186/BF03548186

Bovine Leukocyte Adhesion Deficiency in Danish Holstein-Friesian Cattle

I. PCR Screening and Allele Frequency Estimation

CB Jørgensen 11,21,31,, JS Agerholm 11,21,31, J Pedersen 11,21,31, PD Thomsen 11,21,31,41
PMCID: PMC8112504  PMID: 7906090

Abstract

Jergensen, C. B., J. S. Agerholm, J. Pedersen and P. D. Thomsen: Bovine leukocyte adhesion deficiency in Danish Holstein-Friesian Cattle I. PCR screening and allele frequency estimation. Acta vet. Scand. 1993,34,231-236.–A screening program for bovine leukocyte adhesion deficiency (BLAD) in Danish Holstein-Friesian cattle has been initiated. During the first months 1611 animals were tested by a PCR based assay. Of these animals 1256, 346, and 8 were assigned normal, BLAD carriers, and BLAD affected animals, respectively One bull, born as a co-twin, showed weak reaction for the BLAD allele on DNA isolated from leukocytes, but a normal genotype on DNA isolated from semen. Chromosome analysis showed that this bull was a blood chimaera. Estimation of the BLAD allele frequency upon the PCR test results showed that around 450 Danish calves born in 1991 might have been affected with the recessive disorder.

Keywords: BLAD, hereditary disease

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Acknowledgments

We are grateful to Dr. M.E. Kehrli, Jr., The National Animal Disease Center, USDA-Agricultural Research Service, Ames, Iowa, USA for providing us with the protocol for the PCR assay and to Dr. D. Bailey, University of Cambridge, Cambridge, UK for the Hot-start hint. Thanks to Dr. T. Lykke, The Danish Agricultural Advisory Centre, Aarhus, Denmark for initiating the program. Thanks to Dr. B. Aasted, The Royal Veterinary and Agricultural University, Copenhagen, Denmark for the flow cytometric analysis and to Dr. K. Christensen, The Royal Veterinary and Agricultural University, Copenhagen, Denmark for the chromosome analysis. Last but not least thanks to Mrs. I. Christensen and Mrs. A.B. Nielsen for excellent technical assistance.

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