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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Diabetologia. 2021 Feb 9;64(5):1133–1143. doi: 10.1007/s00125-021-05390-x

Fig. 2.

Fig. 2

Decreased glucose tolerance with age following activation of mutGCK. IPGTT: activation of beta-mutGCK has no effect on glucose tolerance at the age of 1.5 months (a) (n=8 beta-mutGCK and 9 control mice), but resulted in impaired glucose tolerance by the age of 8 months (b) (n=10 beta-mutGCK and 9 control mice). Ex vivo dynamic measurements of insulin secretion, normalised to unmethylated DNA of the insulin 2 gene (perifusion): insulin secretion at age 1.5 months (c) and at 8 months of age (d) (n=6 mice/group). (e) Glucose response to i.p. insulin in mice pre-treated with diazoxide showing similar glucose clearance in WT and beta-mutGCK mice (n=8 beta-mutGCK and 6 control mice). (f) Left, beta cell mass [(pancreas weight × %beta cells)/body weight] in WT and mutant mice at the age of 1.5 months (young) (n=7 mice in each group) and at 8 months of age (old) (n=10 mice in each group). 2.8G, 2.8mmol/l glucose; 16.8G, 16.8 mmol/l glucose; BCM, beta cell mass; unmeth, unmethylated. Bars represent standard error (*p<0.05, **p<0.001).