Skip to main content
PMC home page
Acta Veterinaria Scandinavica logoLink to Acta Veterinaria Scandinavica
. 1992 Mar 1;33(1):1–8. doi: 10.1186/BF03546929

Pathophysiology of Experimental Bovine Endotoxicosis: Endotoxin Induced Synthesis of Prostaglandins and Thromboxane and the Modulatory Effect of Some Non-Steroidal Anti-Inflammatory Drugs

Den eksperimentelle bovine endotoksikoses patofysiologi: Endotoksin-induceret eikosanoidsyntese og den suppressive effekt af nogle non-steroide antiinflammatoriske stoffer

N Jarløv 1, P Haubro Andersen 1,✉, M Hesselholt 1
PMCID: PMC8117851  PMID: 1598851

Abstract

Endotoxin-induced synthesis of thromboxane A2 (TXA2), prostacyclin (PGI2) and prostaglandin E2 (PGE2) was studied in 3 cows after intravenous E. coli endotoxin (055:B5–0.025 mg/kg b.w.) administration. Blood sampling and monitoring of clinical signs were performed from 2 h prior to until 6 h after endotoxin challenge. Blood samples were analyzed for stable hydrolysis products of TXA2 (TXB2), PGI2 (6-keto PGF) and PGE2 (bicyclic PGE2), biochemical and haematological parameters. In a similar experimental design the efficacy of the non-steroidal anti-inflammatory drugs (NSAID) flunixin meglumine (FM) and phenylbutazone (PB) in suppressing eicosanoid synthesis and clinical signs in response to endotoxin challenge was investigated. Two groups of cows, each comprising 2 animals, were treated with FM and PB prior to endotoxin challenge. It was observed that plasma concentrations of TXB2, 6-keto PGF and bicyclic PGE2 increased rapidly after endotoxin challenge. Concentrations were significantly elevated for hours and were correlated to the severity of clinical signs of endotoxicosis. Pretreatment with NSAID suppressed mediator production and alleviated clinical signs. The experiments suggest a certain pathophysiological role of TXA2, PGI2 and PGE2 for the early systemic ill-effects of bovine endotoxicosis.

Keywords: eicosanoids, prostacyclin, flunixin meglunine, phenylbutazone

Full Text

The Full Text of this article is available as a PDF (968.2 KB).

References

  1. Haubro Andersen P, Jarløv N, Hesselholt M. Pathophysiology of experimental bovine endotoxicosis I: Endotoxin in vivo clearance in healthy and hepatic lipidotic cattle. Proceedings XVth World Buiatrics Congress. 1988. pp. 1410–1415. [Google Scholar]
  2. Anderson K L, Smith A R, Shanks D, Davis L E, Gustafsson B K. Efficacy of flunixin meglumine for treatment of endotoxin-induced bovine mastitis. Amer. J. vet. Res. 1986;47:1366–1372. [PubMed] [Google Scholar]
  3. Bottoms G D, Templeton C B, Fessier J F, Johnson M A, Roesel O F, Ewert K M, Adams S B. Thromboxane, prostaglandin I2 (epoprostenol), and the hemodynamic changes in equine endotoxin shock. Amer. J. vet. Res. 1982;43:999–1002. [PubMed] [Google Scholar]
  4. Bottoms DG, Johnson M A, Roesel O F. Endotoxin-induced hemodynamic changes in dogs: Role of thromboxane and prostaglandin I2. Amer. J. vet. Res. 1983;44:1497–1500. [PubMed] [Google Scholar]
  5. Bult H, Beetens J, Vercruysse P, Herman A G. Endotoxin-induced hypotension and blood levels of 6-keto-prostaglandin Fl alpha. Samuelsson & Ramwell (ed.): Adv. PG and TX Res. Raven Press, NY. 1980;7:839–841. [PubMed] [Google Scholar]
  6. Demers L M, Derck D D. A radioimmunoassay for 6-keto-prostaglandin F1 alpha. Samuelsson & Ramwell, (ed.): Adv. PG and TX Res. Raven Press, NY. 1980;7:193–199. [PubMed] [Google Scholar]
  7. Dunkle N J, Bottoms G D, Fessier J F, Knox K, Roesel O F. Effects of flunixin-meglumine on blood pressure and fluid compartment volume changes in ponies given endotoxin. Amer. J. vet. Res. 1985;46:1540–1544. [PubMed] [Google Scholar]
  8. Dyerberg J. N-3 poly-unsaturated fatty acids and their possible role in the prophylaxis of disease. A review with particular attention to ischaemic heart disease. Ugeskr. Læger. 1987;149:1723–1728. [PubMed] [Google Scholar]
  9. Gota Y, Baez S, Orkin L R. The effects of endotoxin on microcirculation and lymphatic dynamics in the rat. Circ. Shock. 1981;8:533–542. [PubMed] [Google Scholar]
  10. Granstrøm, E, Kindahl H, Samuelsson B: Radioimmunoassay for Thromboxane B2. Samuelsson & Ramwell (ed.): Adv. PG &TX Res. Raven Press, NY. 1980, 193–199.
  11. Harris R H, Zmudka M, Maddox Y, Ramwell PW, Fletcher J R. Relationships of TXB2 and 6-keto PGF1 alfa to the hemodynamic changes during baboon endotoxic shock. Samuelsson & Ramwell (ed.): Adv. PG & TX Res. Raven Press, NY. 1980;7:843–849. [PubMed] [Google Scholar]
  12. Hsush W, Gonzalez-Crussi F, Arroyave J L. Platelet-activating factor: An endogenous mediator for bowel necrosis in endotoxemia. FASEB J. 1987;1:403–405. doi: 10.1096/fasebj.1.5.3678700. [DOI] [PubMed] [Google Scholar]
  13. Moore NJ, Hardee M M, Hardee G E. Modulation of arachidonic acid metabolism in endotoxic horses: Comparison of flunixin meglumine, phenylbutazone, and a selective thromboxane synthetase inhibitor. Amer. J. vet. Res. 1986;47:110–113. [PubMed] [Google Scholar]
  14. Morris D D, Bottoms G D, Whitlock R H, Johnson M A. Endotoxin-induced changes in plasma concentrations of thromboxane and prostacyclin in neonatal calves given antiserum to a mutant E.coli (j-5) Amer. J. vet. Res. 1986;47:2520–2524. [PubMed] [Google Scholar]
  15. Powell W S. Rapid extraction of oxygenated metabolites of arachidonic acid from biological samples using octadecylsilyl silica. Prostaglandins. 1980;20:947–957. doi: 10.1016/0090-6980(80)90144-6. [DOI] [PubMed] [Google Scholar]
  16. Salmon J A, Flower R J: Prostaglandins and related compounds. In: Gry & James (ed.): Hormones in Blood. Academic Press 1983, 137–165.
  17. Schrauwen E, Vandeplassche G, Laekemann G, Houvenaghel A. Endotoxin shock in the pig: Release of prostaglandins and beneficial effects of flurbiprofen. Arch. Int. Pharmacodyn. 1983;262:332–334. [PubMed] [Google Scholar]

Articles from Acta Veterinaria Scandinavica are provided here courtesy of BMC

RESOURCES