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. 2021 Apr 19;11(13):6461–6476. doi: 10.7150/thno.54917

Figure 1.

Figure 1

Transcriptionally distinct subtypes in human dermal ECs via scRNA-seq. (A) Workflow. Skin tissues from 10 healthy donors were used for scRNA-seq. CD31+ cell enriched by MACS were further purified to select live CD31+ CD45- cells by FACS. 10X Genomics Chromium scRNA-seq was used to profile the cells. (B) UMAP plot of 33,265 human dermal endothelial cells, colored by cluster. (C) Expressions of SEMA3G, PLVAP, SELE, ACKR1, FBLN2, LYVE1, and PROX1 in each cluster. y axis represents log-normalized expression. (D) Heatmap of single-cell expressions of the top-10 DEGs in each cluster. Cluster A: arteriole ECs; Cluster C1, C2: capillary ECs; Cluster P: post-capillary ECs; Cluster V: venule ECs; Cluster L1, L2: lymphatic endothelial cells. A.U.: arbitrary unit; DEGs: differentially expressed genes; ECs: vascular endothelial cells; FACS: fluorescence activated cell sorting; MACS: magnetic-activated cell sorting; scRNA-seq: single-cell RNA sequencing; UMAP: uniform manifold approximation and projection.