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. 2021 Jan 22;162(6):1816–1827. doi: 10.1097/j.pain.0000000000002169

Figure 2.

Figure 2.

Recombination in the glabrous skin, cutaneous nerve, and dorsal root ganglia after hydroxy tamoxifen painting in Sox10-TOM mice. (A) Recombination in the glabrous skin of vehicle-painted and hydroxy tamoxifen (OH-Tx)-painted paw in Sox10-TOM mice. Immunohistochemistry for TOMATO, S100β, and Sox10. Recombination is evident in OH-Tx-painted paw, whereas no recombination at all was observed in vehicle-painted paw. Arrows indicate Schwann cell nucleus. Dashed line indicates dermal–epidermal border. Scale bar: 20 µm. (B) Quantification of recombination in glabrous skin as represented by % of TOM+SOX10+ cells to total SOX10+ cells in vehicle-painted and OH-Tx-painted paw (n = 165 cells in vehicle treatment and n = 170 cells in OH-Tx, n = 2 animals per treatment). Two-tailed unpaired t test with Welch correction. P < 0.0001. Data are presented as mean ± standard error of the mean. (C) Recombination in the median palmar nerve of vehicle-painted and OH-Tx-painted paw in Sox10-TOM mice. Immunohistochemistry for TOMATO and Sox10. No recombination at all was observed in both OH-Tx- and vehicle-painted paw. Scale bar: 20 µm. (D) Recombination in the dorsal root ganglion of vehicle-painted and OH-Tx-painted paw in Sox10-TOM mice. Immunohistochemistry for TOMATO and Sox10. No recombination at all was observed in both OH-Tx- and vehicle-painted paws. Scale bar: 20 µm.