a-f, Fraction purity, viability, and yield for (a) MC38 (n=5 mice), (b) CT26 (n=4 mice), and (c) Renca (n=4 mice) subcutaneous tumors; (d) intrarenal Renca tumors (n=3 mice); (e) AOM/DSS-induced CRC tumors (n=6 for tumors, n=11 mice for spleens); and (f) spontaneous PyMT GEMM (n=3 mice) tumors. g, Representative flow cytometry analysis of PyMT and AOM/DSS CRC whole tumor, CD45+ immune cell, and EPCAM+ cancer cell fractions gated on live cells. Each data point represents a biological replicate and graphs show mean and SEM. Data are representative studies performed independently at least twice. AOM/DSS CRC: azoxymethane/dextran sodium sulfate-induced colorectal cancer; GEMM: genetically engineered mouse model; PyMT: polyoma virus middle T antigen.