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. 2021 Apr 22;26(9):2434. doi: 10.3390/molecules26092434

Figure 6.

Figure 6

CT extract reduces in vitro lipid accumulation in AML-12 mice hepatocyte cells. (a) MTT assay was performed in AML-12 cell treated with whole CT leaf extract (100–400 µg/mL) and kaempferol (100–400 µM). (b) AML-12 cells were seeded, after inducing steatosis by 1 mmol/L (3:1, oleic acid: palmitic acid) medium, cells were treated with 0.1% DMSO or CT extract (200 and 400 µg/mL) or kaempferol (50 and 100 µM) and performed Oil Red O staining (left) and absorbance (right) was observed at 500 nm. Intracellular ROS was estimated by dihydroethidium (DHE) staining in AML12-cell treated with (c) CT extract (d) kaempferol and bar graph was plotted for fluorescence intensities of DHE (each right side). (e) The expression level of heme oxigenase-1 (HO-1) and nuclear factor, erythroid 2 like 2 (Nrf2) were performed by western blotting and normalized to β-actin. The result is representative of three individual experiments. Values are mean ± SD. AVOVA p-value: ** <0.01 vs. control group; ## <0.01 vs. DMSO treated group.