Abstract
A modified technique for production of antigen and performance of the test is described. A suspension of infected neutrophils was directly applied to multiwell slides. Multichannel pipettes may be used for dilution and application of sera. The modification inreases the capacity both by production of the antigen and by performance of the test. This paper also gives a quantitative determination of the antibodies.
Keywords: tick-borne fever
Sammendrag
En modifisert teknikk for produksjon av antigen og utførelse av testen er beskrevet. En suspension med infiserte nøytrofile granulocytter avsettes direkte på objektglass med 10 teflonbrønner. Multikanal pipetter kan nyttes til fortynning og applikasjon av sera. Modifíkasjonen øker kapasiteten både ved produksjon av antigen og ved utførelsen av testen. Artikkelen inneholder også en kvantitativ bestemmelse av antistoffer.
Full Text
The Full Text of this article is available as a PDF (554.2 KB).
References
- Carlson GP, Kaneko JJ. Isolation of leucocytes from bovine peripheral blood. Proc. Soc. exp. Biol. Med. 1972;142:853–856. doi: 10.3181/00379727-142-37131. [DOI] [PubMed] [Google Scholar]
- Christensson D. Improvement of the teflonized slides used in the immunofluorescent antibody technique. Acta vet. scand. 1986;27:296–298. doi: 10.1186/BF03548173. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Foggie A, Lumsden WHR, McNeillage JC: Preservation of the infectious agent of tick-borne fever in the frozen state. J. comp. Path. 1966, 76, 413. [DOI] [PubMed]
- Paxton EA, Scott GR. Detection of antibodies to the agent of tick-borne fever by indirect immunofluorescence. Vet. Microbiol. 1989;21:133–138. doi: 10.1016/0378-1135(89)90025-4. [DOI] [PubMed] [Google Scholar]
- Scott GR. Tick-borne fever in sheep. Vet. Ann. 1984;24:100–106. [Google Scholar]
- Snodgrass DR, Ramachandran S. A complement fixation test for tick-borne fever of sheep. Br. vet. J. 1971;127:44–46. doi: 10.1016/S0007-1935(17)37382-7. [DOI] [PubMed] [Google Scholar]
- Tuomi J. Experimental studies on bovine tickborne fever (4) Immunofluorescent staining of the agent and demonstration of antigenic relationship between strains. Acta path, microbiol. scand. 1967;71:101–108. [Google Scholar]
- Webster KA, Mitchell GBB. Use of counter immunoelectrophoresis in detection of antibodies to tickborne fever. Res. Vet. Sci. 1988;45:28–30. doi: 10.1016/S0034-5288(18)30890-7. [DOI] [PubMed] [Google Scholar]