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. 1990 Mar 1;31(1):97–107. doi: 10.1186/BF03547582

Experimentally Induced Testicular Alterations in Boars: Hormonal Changes in Mature and Peripubertal Boars

Experimenteilt framkallad testikeldegeneration hos galt: hormonella förändringar hos könsmogna och pubertala gaitar.

Lena Malmgren 1,✉
PMCID: PMC8133200  PMID: 2169189

Abstract

Malmgren, L.: Experimentally induced testicular alterations in boars: Hormonal changes in mature and peripubertal boars. Acta scand. vet. 1990, 31, 97–107. - Eleven sexually mature boars and 10 peripubertal boars were used to study the effects of elevated testicular temperature on plasma hormonal levels. The scrotum of the boars was covered with a textile-aluminium foil insulation device for 100 h. Insulation of the scrotum in the peripubertal boars took place at an age of 100 days.

Blood samples were drawn 3 times daily for 12 days in the mature boars, starting 3 days before scrotal insulation. In the peripubertal boars, blood sampling was performed once a day for 11 days, starting the first day of scrotal insulation.

During scrotal insulation, the plasma levels of testosterone, oestradiol-17 β and oestrone sulphate decreased continuously in the mature boars. After removal of the scrotal insulation device there was a continuously increase, back to normal levels of oestradiol-17 β and oestrone sulphate. The plasma levels of testosterone showed an immediate rise of brief duration after removal of the device in 5 of the boars, while in the other 6 boars the rise in testosterone levels came 4 days after removal and lasted for 3 days.

In the peripubertal boars, there were no significant differences in the hormone levels between the experimental and control animals during and after scrotal insulation. However, the decrease in testosterone concentration over time, during scrotal insulation, was significant within the experimental group.

Keyword: Boar, mature, peripubertal, testosterone, oestradiol-17 β, oestrone sulphate

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References

  1. Aakvaag A, Bentdal O, Quigstad K, Walstad P, Ronningen H, Fonnum F. Testosterone and testosterone binding globulin (TeBG) in young men during prolonged stress. Int. J. Androl. 1978;1:22–31. [Google Scholar]
  2. Allrich RD, Christenson RK, Ford JJ, Zimmerman DR. Pubertal development of the boar: testosterone, estradiol-17β, Cortisol and LH concentrations before and after castration at various ages. J. Anim. Sci. 1982;55:1139–1146. doi: 10.2527/jas1982.5551139x. [DOI] [PubMed] [Google Scholar]
  3. Berg A, Damber JE. Morphometric and functional investigation on the Leydig cells in experimental unilateral cryptorchism in the rat. Int. J. Androl. 1978;7:549–562. [Google Scholar]
  4. Boilert B, Edqvist L-E, Johansson EDB, Lindberg P, Martinsson K. The influence of conjugated estrogens in radioimmunoassays using different antibodies against oestradiol-17β. Steroids. 1973;22:891–894. doi: 10.1016/0039-128x(73)90060-3. [DOI] [PubMed] [Google Scholar]
  5. Brock LW, Wetteman RP. Variations in serum testosterone in boars. J. Anim. Sci. (Abstr.) 1976;42:244–245. [Google Scholar]
  6. Claus R, Gimenez T. Diurnal rhythm of 5α-androst-16-en-3-one and testosterone in peripheral plasma of boars. Acta Endocr. 1977;84:200–206. doi: 10.1530/acta.0.0840200. [DOI] [PubMed] [Google Scholar]
  7. Damber J-E, Berg A, Janson PO. Testicular blood flow and testosterone concentrations in the spermatic venous blood in rats with experimental cryptorchidism. Acta Endocr. 1978;88:611–618. doi: 10.1530/acta.0.0880611. [DOI] [PubMed] [Google Scholar]
  8. Dorrington JH, Fritz IB, Armstrong DT. Control of testicular estrogens synthesis. 1978;18:55–64. doi: 10.1095/biolreprod18.1.55. [DOI] [PubMed] [Google Scholar]
  9. Edqvist LE, Einarsson S, Larsson K, Lundström K. Diurnal variations in peripheral plasma levels of testosterone, androstenone and Cortisol in boars. Acta vet. scand. 1980;21:451–453. doi: 10.1186/BF03546878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Florcruz SV, Lapwood KR. A longitudinal study of pubertal development in boars. Int. J. Androl. 1978;I:317–330. [Google Scholar]
  11. Ford JJ. Serum estrogen concentrations during postnatal development in male pigs. Proc. The Soc. Exper. Biol. and Med. 1983;174:160–164. doi: 10.3181/00379727-174-41719. [DOI] [PubMed] [Google Scholar]
  12. Gomes WR, Butler WR, Johnson AD. Effect of elevated ambient temperature on testis and blood levels and in vitro biosynthesis of testosterone in the ram. J. Anim. Sci. 1971;33:804–807. doi: 10.2527/jas1971.334804x. [DOI] [PubMed] [Google Scholar]
  13. Hagenäs L, Ritzen EM. Impaired Sertoli cell function in experimental cryptorchidism in the rat. Mol. Cell. Endocr. 1976;4:25–34. doi: 10.1016/0303-7207(76)90004-6. [DOI] [PubMed] [Google Scholar]
  14. Holst S. Sterility in boars. Nord. Vet.-Med. 1949;1:87–120. [Google Scholar]
  15. Huang HFS, Nieschlag E. Alteration of free sulphydryl content of rat sperm heads by suppression of intratesticular testosterone. J. Reprod. Fert. 1984;70:31–38. doi: 10.1530/jrf.0.0700031. [DOI] [PubMed] [Google Scholar]
  16. Juniewicz PE, Johnson BH. Influence of adrenal steroids upon testosterone secretion by the boar testis. Biol. Reprod. 1981;25:725–733. doi: 10.1095/biolreprod25.4.725. [DOI] [PubMed] [Google Scholar]
  17. Kattesh HG, Knight JW, Gwazdauskas FC, Kornegay ET. Daily alterations in plasma testosterone in boars at different ages. Theriogenology, 1982;18:113–118. doi: 10.1016/0093-691x(82)90055-3. [DOI] [PubMed] [Google Scholar]
  18. Lapwood KR, Florcruz SV. Luteinizing hormone and testosterone secretory profiles of boars: effects of stage of sexual maturation. Theriogenology, 1978;10:293–306. [Google Scholar]
  19. Larsson K, Einarsson S, Lundström K, Hakkarainen J. Endocrine effects of heat stress in boars. Acta, vet. scand. 1983;24:305–314. doi: 10.1186/BF03546734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lindberg BS, Lindberg P, Martinsson K, Johansson EDB: Radioimmunological methods for the estimation of oestrone, oestradiol-17β and oestriol in pregnancy plasma. Acta Obstet. Gynaecol. 1974, Suppl. 32, p 5–19. [DOI] [PubMed]
  21. Liptrap RM, Raeside JI. Increase in plasma testosterone concentration after injection of adrenocorticotrophin into the boar. J. Endocr. 1975;66:123–131. doi: 10.1677/joe.0.0660123. [DOI] [PubMed] [Google Scholar]
  22. Malmgren L, Larsson K. Experimentally induced testicular alterations in boars; histological and ultrastructural findings. J. Vet. Med. Serie A. 1989;36:3–14. doi: 10.1111/j.1439-0442.1989.tb00696.x. [DOI] [PubMed] [Google Scholar]
  23. Malmgren L. Experimentally induced testicular alterations in boars; sperm morphology changes in mature and peripubertal boars. J. Vet. Med. Serie A. 1989;36:411–420. doi: 10.1111/j.1439-0442.1989.tb00748.x. [DOI] [PubMed] [Google Scholar]
  24. Malmgren L: Experimentally induced testicular alterations in boars. Thesis, Uppsala, 1988.
  25. Mazzarri G, du Mesnil, du Buisson F, Ortavant R. Action of temperature and light on spermatogenesis, sperm production and fertility of the boar. Proc. VIth Int. Congr. Anim. Reprod. and A. I. Paris. 1968;1:305–308. [Google Scholar]
  26. Oltner R, Lundström K, Edqvist L-E. LH and testosterone in monozygous growing bulls. Swedish J. agric. Res. 1979;9:151–161. [Google Scholar]
  27. Raeside JI, Renaud RL. Estrogen and androgen production by purified Leydig cells of mature boars. Biol. Reprod. 1983;28:727–733. doi: 10.1095/biolreprod28.3.727. [DOI] [PubMed] [Google Scholar]
  28. Rodriguez H, Kunavongkrit A: Chronical venous catheterization for frequent blood sampling in unrestrained pigs. Acta vet. scand. 1983, 24, 318–320. [DOI] [PMC free article] [PubMed]
  29. SAS Institute Inc.: SAS user’s guide, SAS Institute Inc. Cary, N.C. 1985.
  30. Setchell B: The mammalian testis. Elek Books limited. London. 1978, p. 109–180.
  31. Skinner JD, Rowson LE. Some effects of unilateral cryptorchism and vasectomy on sexual development of the pubescent ram and bull. J. Endocr. 1968;42:311–321. doi: 10.1677/joe.0.0420311. [DOI] [PubMed] [Google Scholar]
  32. Wallgren M, Kindahl H, Larsson K. Clinical, endocrinological and spermatological studies after endotoxin in the ram. J. Vet. Med. Serie A. 1989;36:90–103. doi: 10.1111/j.1439-0442.1989.tb00708.x. [DOI] [PubMed] [Google Scholar]
  33. Wetteman RP, Desjardins C. Testicular function in boars exposed to elevated ambient temperature. Biol. Reprod. 1979;20:235–241. doi: 10.1095/biolreprod20.2.235. [DOI] [PubMed] [Google Scholar]
  34. Wright K, Collins DC, Musey PI, Preedy JRK: A specific radioimmunoassay for estrone sulphate in plasma and urine without hydrolysis. J. clin. Endocr. Metab. 1978, 47, 1092–1098. [DOI] [PubMed]

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