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. Author manuscript; available in PMC: 2021 Sep 9.
Published in final edited form as: Immunity. 2021 Mar 9;54(3):499–513.e5. doi: 10.1016/j.immuni.2021.02.002

Figure 5. Enteric neurons modulate RORγ+ Treg induction through IL6.

Figure 5.

A. Representative flow cytometry profiles after 72 hrs of iTreg cultures (soluble αCD3 + splenic APCs) stained for FoxP3 and RORγ. Each dot is an independent culture. Plot at right compiled from 3 independent experiments.

B. Comparison of MMP neuron SN and recombinant IL6. Top panels: iTreg cultures (soluble αCD3 + splenic APCs) supplemented with titrated neuronal SN in which IL6 concentration had been pre-determined by ELISA. Bottom panels: parallel cultures supplemented with matching concentrations of rIL6 (after ELISA determination). Representative of two independent experiments.

C. Data from B, plotting numbers of RORγ+ and RORγ- Treg cells in iTreg cultures (soluble αCD3 + splenic APCs) supplemented with titrated amounts of neuron supernatant or recombinant IL6. Each dot is the average value of 2 or more independent preparations, plot compiled from 2 independent experiments.

D. Representative flow cytometry profiles of iTreg cultures (soluble αCD3 + splenic APCs) supplemented with MMP neuronal SN and anti-IL6 (Each dot is an independent culture, compiled data from 3 independent experiments plotted at right).

E. As D, where the neuron culture SN was from Il6-deficient mice or control littermates. (Compiled data from 3 independent experiments plotted at right).