Vert, G., Grotz, N., Dédaldéchamp, F., Gaymard, F., Guerinot, M.L., Briat, J.F., and Curie, C. (2002). IRT1, an Arabidopsis Transporter Essential for Iron Uptake from the Soil and for Plant Growth. Plant Cell 14: 1223-1233.
The original western blot shown in Figure 1C monitoring IRT1 protein accumulation in wild-type or irt1-1 knock-out mutant, in both roots and shoots, and in response to iron nutrition was cropped and reassembled to present the data in a more logical way. This unfortunately led to a mistake during figure mounting, with the duplication of part of the blot and mis-annotation of the lanes. The original and corrected Figure 1 are shown below. The results and figure legend are unaltered by this correction.
Figure 1.
Molecular Characterization of the irt1-1 Knockout Mutant. (C) Immunoblot analysis of IRT1. Total proteins were extracted from wild-type and irt1-1 plants grown in the presence (+) or absence (-) of iron. The blot was probed with antibodies directed against IRT1. R, roots; S, shoots.
The northern blots shown in Figure 10A and 10E were made by assembling separate images from two different blots (panel A) or by cropping and reassembling the original scan to remove unnecessary conditions and organs (panel E). We apologize for not having clearly mentioned this in the legend to the corresponding figure and for not having clearly highlighted this in the figure itself. We provide the original source blot corresponding to panel E, to show how the final panel was made. We could not locate the original blots used to build panel A and apologize to the reader for this. We now provide a corrected version of Figure 10 clearly highlighting that panel A and E are composite. The legend of Figure 10 and conclusions remain unchanged.
Figure 10.
IRT1 expression in flower. (A) Spatial distribution of IRT1 expression using 10 µg of total RNA isolated from roots (R), rosette leaves (rL), cauline leaves (cL), floral stalk (fS), flowers (F), and siliques (S) of 6 week-old soil-grown plants. (E) Analysis of IRT1 expression in response to iron status. Gel blot analysis was performed using 15 µg of total RNA isolated from roots (R) and flowers (F) of 6 week-old plants irrigated with either water (H2O) or 0.5 g/L Sequestrene (Fe).
All authors are in agreement with the corrections made. We apologize for any inconvenience this may have caused to the readers.
Note: The corrected figures and accompanying text were reviewed by members of The Plant Cell editorial board. The authors are responsible for providing a complete listing and accurate explanations for all known errors or instances of inappropriate data handling or image manipulation associated with the original publication.