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. 2021 May 20;10(5):43. doi: 10.1038/s41389-021-00330-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Primeview human gene expression array was performed to identify the differentially expressed genes (DEGs) between shPSMC2 and shCtrl groups of GBC-SD cells. B A PSMC2-induced interaction network was established based on IPA analysis. qPCR (C) and western blotting (D) were used to detect the expression of several selected DEGs in GBC-SD cells with or without PSMC2 knockdown. E The expression of GNG4 in GBC tissues and normal tissues was evaluated by IHC analysis. F Kaplan–Meier survival analysis showed that high expression of GNG4 predicts poor prognosis of GBC patients. G The mRNA expression of GNG4 in GBC cell lines was detected by qPCR. The representative images were selected from at least 3 independent experiments. H Co-IP assay was performed to show the direct interaction between PSMC2 and GNG4. Data were shown as mean ± SD. **P < 0.01, ***P < 0.001.