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. 1989 Dec 1;30(4):465–474. doi: 10.1186/BF03548024

Effects of Systematic Influences and Intramammary Infection on Differential and Total Somatic Cell Counts in Quarter Milk Samples from Dairy Cows

Paul Wever 1, Ulf Emanuelson 1,✉
PMCID: PMC8142161  PMID: 2640784

Abstract

Effects of bacteriological status, stage of lactation, parity and season of sampling on differential and total somatic cell counts were estimated in quarter milk samples taken from 39 dairy cows. Log somatic cell count was affected by the bacteriological status of the quarter, as well as by the bacteriological status of adjacent quarters. Differential cell counts were affected by presence or absence of pathogens in the quarters themselves, but not by the bacteriological status of the adjacent quarters. Log somatic cell count was clearly affected by stage of lactation, due mainly to physiological variation, but possibly also accentuated by variation in infection rates throughout lactation. With the exception of early lactation, little physiological variation throughout lactation was detected for differential cell counts. Presence of infections seemed to have some indirect effect on trends throughout lactation as regards percentages of granulocytes and monocytes. Variation in somatic cell counts due to parity could be explained by variation in infection rates, rather than being physiologically determined.

Keywords: physiological effects, bacteriological status, leukocytes, bovine mastitis

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Acknowledgments

The senior author expresses his thanks to the Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, and to Dr. Jan Philipsson for support of his work.

References

  1. Blackburn PS. The variation in the cell count of cow’s milk throughout lactation and from one lactation to the next. J. Dairy Res. 1966;33:193–198. doi: 10.1017/S0022029900011857. [DOI] [Google Scholar]
  2. Dulin AM, Paape MJ, Weinland BT. Effect of parity, stage of lactation and intramammary infection on concentrations of somatic cells and cytoplasmic particles in goat milk. J. Dairy Sci. 1983;66:2426–2433. doi: 10.3168/jds.S0022-0302(83)82101-8. [DOI] [PubMed] [Google Scholar]
  3. Emanuelson U, Olsson T, Matilla M, Äström G, Holmberg O. Effects of parity and stage of lactation on adenosine-triphosphate, somatic cell counts and anti-trypsin content in cow’s milk. J. Dairy Res. 1988;55:49–55. doi: 10.1017/S002202990002584X. [DOI] [PubMed] [Google Scholar]
  4. Emanuelson U, Persson E. Studies on somatic cell counts in milk from Swedish dairy cows. I. Non-genetic causes of variation in monthly test-day results. Acta agric. Scand. 1984;34:33–44. [Google Scholar]
  5. Fox LK, Shook GE, Schultz LH. Factors related to milk loss in quarters with low somatic cell counts. J. Dairy Sci. 1985;68:2100–2107. doi: 10.3168/jds.S0022-0302(85)81074-2. [DOI] [PubMed] [Google Scholar]
  6. Guidry AJ, Paape MJ, Pearson RE. Effects of parturition and lactation on blood and milk cell concentrations, corticosteroids and neutrophil phagocytosis in the cow. Amer. J. vet. Res. 1976;37:1195–1200. [PubMed] [Google Scholar]
  7. Hageltorn M, Saad AM. Flow cytofluorometric characterisation of bovine blood and milk lymphocytes. Amer. J. vet. Res. 1986;47:2012–2016. [PubMed] [Google Scholar]
  8. Hoare RJT, Sheldrake RF, McGregor CD, Woodhouse EW. Somatic cell volume distribution as an aid to the diagnosis of mastitis. J. Dairy Res. 1980;47:167–176. doi: 10.1017/S0022029900021038. [DOI] [PubMed] [Google Scholar]
  9. Honkanen-Buzalski T, Kangasniemi R, Atroshi F, Sandholm M. Effect of lactation stage and number on milk albumin (BSA) and somatic cell count. Zentralbl. Vet. Med. A. 1981;28:760–767. doi: 10.1111/j.1439-0442.1981.tb01248.x. [DOI] [PubMed] [Google Scholar]
  10. Jain NC. Neutrophil leucocytes and inflamation of the bovine mammary gland. Theriogenology. 1976;6:153–173. doi: 10.1016/0093-691X(76)90011-X. [DOI] [Google Scholar]
  11. Jain NC, Schalm DW, Lasmanis J. Experimentally induced coliform (Aerobacter aerogenes) mastitis in normal cows and cows made neutrophenic by an equine anti-bovine leucocyte serum. Amer. J. vet. Res. 1971;32:1929–1935. [PubMed] [Google Scholar]
  12. Jensen DL, Eberhart RJ. Macrophages in bovine milk. Amer. J. vet. Res. 1975;37:619–624. [PubMed] [Google Scholar]
  13. Kennedy BW, Sethar MS, Tong AKW, Moxley JE, Downey BR. Environmental factors influencing test-day somatic cell counts in Holsteins. J. Dairy Sci. 1982;65:275–280. doi: 10.3168/jds.S0022-0302(82)82188-7. [DOI] [Google Scholar]
  14. Klastrup O, Schmidt-Madsen P. Nordiske rekom-mendationer vedrorende mastitisundersogelser av kirtelprover (Nordic recommendations for mastitis investigations on quarter milk samples) Nord. Vet-Med. 1974;26:197–204. [PubMed] [Google Scholar]
  15. Kurzhals P v, Klima H, Manz D. Beziehungen zwischen Zellzahl, Zellbild und bacteriologischen Befunden bei der subklinische Mastitis der Rinder (Relations between cell count, cell pattern and bacteriological findings in bovine subclinical mastitis) Milchwissenschaft. 1985;40:6–9. [Google Scholar]
  16. Lee CH, Wooding FBP, Kemp P. Identification, properties and differential counts of cell populations using electron microscopy of dry cows secretions, colostrum and milk from normal cows. J. Dairy Res. 1980;47:39–50. doi: 10.1017/S0022029900020860. [DOI] [PubMed] [Google Scholar]
  17. McDonald JS, Anderson A J. Total and differential somatic cell counts from noninfected bovine mammary glands: The early non-lactating period. Amer. J. vet. Res. 1981;42:1360–1365. [PubMed] [Google Scholar]
  18. McDonald JS, Anderson A J. Total and differential somatic cell counts in secretions from noninfected bovine mammary glands: The peripartum period. Amer. J. vet. Res. 1981;42:1366–1370. [PubMed] [Google Scholar]
  19. Paape MJ, Wergin A J, Guidry AJ, Pearson RE. Leucocytes - second line of defence against invading mastitis pathogens. J. Dairy Sci. 1979;62:135–153. doi: 10.3168/jds.S0022-0302(79)83215-4. [DOI] [PubMed] [Google Scholar]
  20. SAS Institute Inc. SAS User’s Guide: Statistics. Cary, N.C., USA: SAS Institute Inc.; 1985. The GLM procedure. [Google Scholar]
  21. Schulze WD, BramleyAJ Effect of Escherichia coli endotoxin-mediated inflammation of one mammary quarter of the bovine udder on diapedesis into other quarters. J. Dairy Res. 1982;66:381–385. doi: 10.1017/S0022029900022524. [DOI] [PubMed] [Google Scholar]
  22. Sheldrake RF, Hoare RJT, McGregor GD. Lactation stage, parity, and infection affecting somatic cell counts, electrical conductivity and serum albumin in milk. J. Dairy Sci. 1983;66:542–547. doi: 10.3168/jds.S0022-0302(83)81823-2. [DOI] [PubMed] [Google Scholar]

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