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. 1989 Jun 1;30(2):141–146. doi: 10.1186/BF03548050

Equine Herpes Virus 1 (EHV-1) in Liver, Spleen, and Lung as Demonstrated by Immunohistology and Electron Microscopy

Lars Jönsson 1,✉, Johan Beck-Friis 1, Lena H M Renström 1, Tapio Nikkilä 1, Per Thebo 1, Bo Sundquist 1
PMCID: PMC8142178  PMID: 2556904

Abstract

Ten aborted foals, diagnosed as infected with Equine Herpes Virus 1 (EHV-1) on histopathological criteria, were examined for the presence of EHV-1 using immunohistology as the investigative instrument. The primary reagent was an antiserum specific for viral envelope glycoproteins. Immunohistology localised EHV-1 to areas of liver necrosis and to the cytoplasm of infected Kupffer cells and hepatocytes. Cytoplasmic immunolabelling was also prominent in reticular cells of the red pulp of the spleen and in intact and degenerated bronchiolar epithelium. Cytoplasmic immunolabelling was seen in morphologically unchanged cells and in cells containing intranuclear inclusion bodies. Three aborted foetuses with no histological signs of EHV-1 infection were negative when immunostained for EHV-1.

Detection by electron microscopy of EHV-1 virions confirmed the EHV-1 specificity of the immunolabelling procedure.

Keywords: equine virus abortion, immunolabelling, tissue sections

Full Text

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Acknowledgments

The authors wish to thank Miss Cajsa Magnusson, Mrs. Lena Ekman and Mr. Bengt Ekberg for technical assistance and Mrs. Ulla Malmström for preparing the manuscript. Prof. Claes Rehbinder and Ass. Prof. Berndt Klingebom are gratefully acknowledged for critically evaluating the results.

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