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. 2021 Apr 21;10(5):500. doi: 10.3390/pathogens10050500

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Dose-sparing effect of Advax and Advax-CpG adjuvant on an experimental TIPV vaccine (SSI). Female BALB/c mice (n = 9/group) received two i.m. immunizations 3 weeks apart with indicated dose of TIPV vaccine with (Grey and Black bars) or without (White bars) Advax or Advax-CpG. Serum neutralizing (NT) titers against type 1—Brunhilde, type 2—MEF-1 and type 3—Saukett were calculated using the Kärber method (Mean + SD). (A). Dose-sparing effect of Advax adjuvant for serotype 1 was further determined by ELISA (B). Antibody values are mean + SD with significance indicated as * p < 0.05, *** p < 0.001 or NS; not-significant compared with 10 DU antigen alone group using Mann–Whitney test.