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. 2021 Apr 23;13(5):742. doi: 10.3390/v13050742

Figure 7.

Figure 7

(a) BPIFA1 Primers were designed to traverse exon-exon junctions to be mRNA specific; (b) BPIFA1 RT-LAMP primer set specificity using New England Biolabs Colorimetric RT-LAMP kit: 1. Nasopharyngeal swab, TRIzol/chloroform extraction 2. Nasopharyngeal swab, Qiagen Viral RNA Mini kit extraction 3. Saliva RNA 4. Blood RNA 5. Nasopharynx flush-through (NFT), Qiagen Viral RNA Mini Kit extraction 6. Human (male) gDNA 7,8. Non-template water control, (c)—BPIFA1 detection across 11 individuals spiked and non-spiked with SARS-Cov-2, 500 PFU of SARS-CoV-2 and non-template water controls.