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. 2021 Apr 28;13(5):783. doi: 10.3390/v13050783

Figure 6.

Figure 6

CRISPRi silencing of latent genes in BCBL-1 cells. (A) Region of the viral genome encoding LANA (ORF73) and position of the sgRNAs. (B) Immunoblot analysis of LANA in iSLK-219 cells, and iSLK-219-dC9K cells transduced with a non-targeting (NT) sgRNA or a LANA specific sgRNA. Image representative of three biological replicates. (C) Quantification of the immunoblot data for sgRNAs that silenced LANA. Error bars: standard deviation (one-way ANOVA, *** p = 0.0004, ** p = 0.004, * p = 0.0124, ns, not significant).