Figure 4.

Two-dimensional SDS-PAGE and western blotting of PrP^res of VPSPr. Two-dimensional (2D) SDS-PAGE and western blotting of PrP^res in brain homogenates of VPSPr was probed with 3F4 (A,C,E,G) or 1E4 (B,D,F,H) antibodies without treatment (A,B), treated with PK alone (C,D), with PNGase F alone (E,F), and with PK along with PNGase F (G,H). 2D of PrP from brain homogenates of VPSPr probed with the anti-N (I) or anti-C (J) antibody without PK and PNGase F treatment. Dotted ovals represent the areas that miss PrP spots on 1E4 or anti-C blots compared to 3F4 or Anti-N antibody.