Table 2.
Parameters of the scheduled MRM method (MRM detection window 40 s; CE = collision energy; CXP = cell exit potential; DP = declustering potential). The product ions are listed in decreasing intensity.
| Marker Peptide | tR [Min] | DP [V] | m/z (Charge State) | Product Ions | CE [V] | CXP [V] | |
|---|---|---|---|---|---|---|---|
| Alfalfa 1 | VEGGLSIMSPPER | 4.91 ± 0.02 | 71 | 686.4 (+2) | 498.3 (y4), 585.3 (y5), 716.3 (y6) | 27/29/31 | 26/26/24 |
| Alfalfa 2 | FNLEAGDIMR | 5.75 ± 0.02 | 56 | 583.3 (+2) | 662.3 (y6), 419.2 (y3), 591.3 (y5) | 29/39/27 | 32/24/30 |
| Alfalfa 3 | ISDVNSLTLPILR | 7.60 ± 0.01 | 60 | 720.9 (+2) | 498.3 (y4), 712.5 (y6), 316.2 (b3) | 35/35/35 | 36/36/36 |
| Broad bean 1 | EDVLSLAPK | 4.77 ± 0.02 | 36 | 486.3 (+2) | 515.3 (y5), 315.2 (y3), 628.4 (y6) | 23/21/25 | 24/16/32 |
| Broad bean 2 | FNLEEGDLIR | 5.90 ± 0.01 | 16 | 603.3 (+2) | 702.4 (y6), 401.3 (y3), 573.3 (y5) | 31/43/31 | 28/22/26 |
| Broad bean 3 | LSPGDVLVIPAGYPVAIK | 8.47 ± 0.01 | 16 | 603.7 (+3) | 458.3 (y92+), 527.4 (y5), 514.8 (y102+) | 21/35/15 | 24/36/36 |
| Chickpea 1 | GGLSFISPSEK | 4.44 ± 0.02 | 11 | 561.3 (+2) | 547.3 (y5), 460.2 (y4), 660.4 (y6) | 27/37/27 | 28/30/30 |
| Chickpea 2 | IVDLAIPINTPAK | 6.53 ± 0.01 | 26 | 682.9 (+2) | 740.4 (y7), 328.2 (b3), 625.4 (b6) | 29/35/25 | 42/22/30 |
| Chickpea 3 | SSNPFTFLVPPR | 7.46 ± 0.01 | 91 | 681.8 (+2) | 369.2 (y3), 468.3 (y4), 581.4 (y5) | 25/33/33 | 24/22/32 |
| Lentil 1 | VILEDQEQEPQHR | 1.87 ± 0.03 | 31 | 540.9 (+3) | 704.8 (y112+), 648.3 (y102+), 470.2 (y113+) | 23/21/23 | 40/42/24 |
| Lentil 2 | FFEVTPEK | 3.93 ± 0.01 | 66 | 498.8 (+2) | 702.4 (y6), 373.2 (y3), 573.3 (y5) | 21/35/25 | 34/28/32 |
| Lentil 3 | VVDFVISLNRPGK | 5.41 ± 0.03 | 101 | 481.9 (+3) | 623.4 (y112+), 301.2 (y3), 884.5 (y8) | 19/37/27 | 44/20/40 |
| Lupine blue 1 | QQEQQLEGELEK [25] | 2.48 ± 0.02 | 36 | 729.9 (+2) | 389.2 (y3), 386.2 (b3), 575.3 (y5) | 37/35/33 | 29/18/28 |
| Lupine blue 2 | NTLEATFNTR [25,31] | 3.35 ± 0.01 | 31 | 583.8 (+2) | 838.4 (y7), 709.4 (y6), 458.2 (b4) | 31/31/21 | 50/40/26 |
| Lupine blue 3 | ISSVNSLTLPILR [25] | 7.11 ± 0.01 | 45 | 706.9 (+2) | 498.3 (y4), 712.5 (y6), 359.2 (a4) | 33/31/35 | 22/30/24 |
| Lupine white 1 | NPYHFSSQR [31] | 0.49 ± 0.06 | 21 | 379.2 (+3) | 341.2 (y83+), 373.7 (b62+), 390.2 (y3) | 17/13/23 | 20/24/20 |
| Lupine white 2 | DKPSQSGPFNLR | 2.83 ± 0.02 | 46 | 449.2 (+3) | 323.7 (y52+), 646.4 (y5), 549.3 (y4) | 19/21/27 | 16/30/40 |
| Lupine white 3 | AVNELTFPGSAEDIER | 5.90 ± 0.01 | 46 | 583.3 (+3) | 487.2 (y92+), 560.8 (y102+), 417.2 (y3) | 21/15/41 | 22/40/22 |
| Pea 1 | ELTFPGSVQEINR [25] | 5.68 ± 0.02 | 41 | 745.4 (+2) | 999.5 (y9), 500.3 (y92+), 491.3 (b4) | 37/37/29 | 48/28/26 |
| Pea 2 | LSSGDVFVIPAGHPVAVK [25] | 5.72 ± 0.02 | 120 | 598.3 (+3) | 438.3 (y92+), 513.3 (y5), 363.2 (y113+) | 27/37/43 | 34/24/24 |
| Pea 3 | LTPGDVFVIPAGHPVAVR [25] | 6.57 ± 0.01 | 36 | 615.7 (+3) | 544.3 (y163+), 541.3 (y5), 452.3 (y92+) | 21/35/33 | 28/38/14 |
| Peanut 1 | GTGNLELVAVR [32,33,34,35,36] | 4.45 ± 0.02 | 96 | 564.8 (+2) | 345.2 (y3), 557.4 (y5), 686.4 (y6) | 29/33/31 | 24/28/38 |
| Peanut 2 | FNLAGNHEQEFLR [32,33,37,38] | 4.45 ± 0.02 | 61 | 525.6 (+3) | 262.1 (b2), 657.3 (y112+), 600.8 (y102+) | 23/23/23 | 14/40/16 |
| Peanut 3 | WLGLSAEYGNLYR [32,34] | 7.02 ± 0.01 | 16 | 771.4 (+2) | 272.2 (a2), 300.2 (b2), 357.2 (b3) | 39/35/39 | 14/18/18 |
| Soy 1 | SQSDNFEYVSFK [23,34,36] | 5.02 ± 0.04 | 31 | 725.8 (+2) | 381.2 (y3), 643.3 (y5), 1235.6 (y10) | 35/35/29 | 26/46/52 |
| Soy 2 | EAFGVNMQIVR [25,34,38,39] | 5.77 ± 0.02 | 61 | 632.3 (+2) | 760.4(y6), 387.3 (y3), 532.3 (y92+) | 29/29/27 | 38/22/34 |
| Soy 3 | FYLAGNQEQEFLK [22,23,25,36] | 6.00 ± 0.01 | 36 | 793.9 (+2) | 311.1 (b2), 424.2 (b3), 638.8 (y112+) | 41/35/33 | 18/26/38 |