Figure 5.

CP12_ox in isolation and in the presence of C. reinhardtii cell extract. (a) ¹H-¹⁵N HSQC spectrum of isolated CP12_ox. (b) Overlay of the ¹H-¹⁵N HSQC spectra of isolated CP12_ox (black) and that of CP12 in the presence of C. reinhardtii cell extract (green, molecular crowding corresponding to 19 mg mL⁻¹ of protein). The data is recorded in the presence of 20 mM DTT_ox, and 0.1 mM DCMU. The assignments of the resonances that are observed in the presence of cell extract are indicated. (c) ¹H-¹⁵N HSQC of CP12 in the presence of C. reinhardtii cell extract collected in the light (molecular crowding corresponding to 15 mg mL⁻¹ of protein) and 20 mM H₂O₂ as a strong oxidizing agent. (d) Signal intensities for all residues in the ¹H-¹⁵N HSQC spectrum of isolated CP12_ox (black), and in the ¹H-¹⁵N HSQC spectrum of CP12_ox in presence of C. reinhardtii cell extract (green). The signal intensities are normalized against the mean of all intensities. The stars indicate residues for which the resonances are broadened beyond detection in isolated CP12_ox [31], and the disulfide bridges are indicated above the sequence. The CP12 sequence is indicated below the graph with same color coding as in Figure 2.