Skip to main content
NCBI home page
Acta Veterinaria Scandinavica logoLink to Acta Veterinaria Scandinavica
. 1988 Jun 1;29(2):219–223. doi: 10.1186/BF03548373

Ochratoxin A as a Suppressor of Mitogen-Induced Blastogenesis of Porcine Blood Lymphocytes

Ochratoxin A som hämmare af mitogen-inducerad blastogenes av lymfocyter från grisar

Torbjörn Holmberg 110,210,✉, Ann Thuvander 110,210, Karl Hult 110,210
PMCID: PMC8152549  PMID: 2975921

Abstract

The in vitro effect of ochratoxin A on pig lymphocytes stimulated by the mitogen Concanavalin A was studied by measuring the rates of 3H-thymidine incorporation in DNA.

Ochratoxin A inhibited the mitogenic response to Concanavalin A in a dose dependent way. An almost total inhibition was obtained with ≥ 1 mg ochratoxin A/1, approximately 60 % inhibition was produced by 0.5 mg ochratoxin A/1 and approximately 10 % inhibition by 0.06 mg ochratoxin A/1.

The immunosuppressive effect of ochratoxin A was not altered much by different contents of bovine serum albumin, 0.1 or 0.3 %, in the cell culture medium.

Keywords: immunosuppression, lymphoblastogenesis, pigs

Full Text

The Full Text of this article is available as a PDF (736.1 KB).

Acknowledgement

This work was supported by grants from the Swedish Council for Forestry and Agricultural Research and from the Foundation of Veterinary Feed Control.

References

  1. Chu FS. Interaction of ochratoxin A with bovine serum albumin. Archs. Biochem. Biophys. 1971;747:359–366. doi: 10.1016/0003-9861(71)90391-2. [DOI] [PubMed] [Google Scholar]
  2. Cooray R. Effects of some mycotoxins on mitogen induced blastogenesis and SCE frequency in human lymphocytes. Fd. Chem. Toxic. 1984;22:529–534. doi: 10.1016/0278-6915(84)90222-9. [DOI] [PubMed] [Google Scholar]
  3. Creppy EE, Störmer FC, Röschenthaler R, Dirheimer G. Effects of two metabolites of ochratoxin A, (4R)-4-hydroxyochratoxin A and ochratoxin a on immune response in mice. Infect. Immun. 1983;39:1015–1018. doi: 10.1128/IAI.39.3.1015-1018.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dwivedi P, Burns RB. Effect of ochratoxin A on immunoglobulins in broiler chicks. Res. Vet. Sci. 1984;36:117–121. doi: 10.1016/S0034-5288(18)32011-3. [DOI] [PubMed] [Google Scholar]
  5. Forsell JH, Pestka JJ. Relation of 8-ketotrichothecene and zearalenone analog structure to inhibition of mitogen-induced human lymphocyte blastogenesis. Appl. Environ. Microbiol. 1985;50:1304–1307. doi: 10.1128/AEM.50.5.1304-1307.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fuchs R, Huit K, Peraica M, Radie B, Plestina R. Conversion of ochratoxin C into ochratoxin A in vivo. Appl. Environ. Microbiol. 1984;48:41–42. doi: 10.1128/AEM.48.1.41-42.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Haubeck H-D, Lorkowski G, Kölsch E, Röschenthaler R. Immunosuppression by ochratoxin A and its prevention by phenylalanine. Appl. Environ. Microbiol. 1981;47:1040–1042. doi: 10.1128/AEM.41.4.1040-1042.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huit K, Hökby E, Gatenbeck S, Rutqvist L. Ochratoxin A in blood from slaughter pigs in Sweden: use in evaluation of toxin content of consumed feed. Appl. Environ. Microbiol. 1980;39:828–830. doi: 10.1128/AEM.39.4.828-830.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Huit K, Rutqvist L, Holmberg T, Thafvelin B, Gatenberg S. Ochratoxin A in blood of slaughter pigs. Nord. Vet. Med. 1984;36:314–316. [PubMed] [Google Scholar]
  10. Huit K, Fuchs R. Analysis and dynamics of ochratoxin A in biological systems. In: Steyn P S, Vleggaar R, editors. Mycotoxins and phycotoxins. 1986. pp. 365–376. [Google Scholar]
  11. Kristensen F, Kristensen B, Lazary S. The lymphocyte stimulation test in veterinary immunology. Vet. Immunol. Immunopathol. 1982;3:203–277. doi: 10.1016/0165-2427(82)90036-8. [DOI] [PubMed] [Google Scholar]
  12. Krogh P. Causal associations of mycotoxic nephropathy. 1978. p. 269. [PubMed] [Google Scholar]
  13. Paul PS, Johnsson DW, Mirocha CJ, Soper FF, Thoen CO, Muscoplat GG, Weber AF. In vitro stimulation of bovine peripheral blood lymphocytes: Suppression of phytomitogen and specific antigen lymphocyte responses by aflatoxin. Amer. J. Vet. Res. 1977;38:2033–2035. [PubMed] [Google Scholar]
  14. Prior MG, Sisodia CS. The effects of ochratoxin A on the immune response of swiss mice. Can. J. comp. Med. 1982;46:91–96. [PMC free article] [PubMed] [Google Scholar]
  15. Röschenthaler R, Creppy EE, Dreismann H, Dirheimer G. Proc. Mycotox. Symp. 1984. Ochratoxin A - on the mechanism of action. In Toxigenic fungi - their toxins and health hazard; pp. 255–264. [Google Scholar]
  16. Szczech GM, Carlton WW, Tuite J. Ochratoxicosis in Beagle dogs II. Pathology. Vet. Pathol. 1973;10:219–231. doi: 10.1177/030098587301000304. [DOI] [PubMed] [Google Scholar]
  17. Szczech GM, Carlton WW, Tuite J, Caldwell R. Ochratoxin A Toxicosis in Swine. Vet. Pathol. 1973;10:347–364. doi: 10.1177/030098587301000408. [DOI] [PubMed] [Google Scholar]

Articles from Acta Veterinaria Scandinavica are provided here courtesy of BMC

RESOURCES