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. 2021 May 13;13(5):711. doi: 10.3390/pharmaceutics13050711

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cytotoxicity analysis of functionalized nanomaterials. (a) Fibroblasts viability analyzed up to 10 days of cell culture using Presto Blue assay for all types of samples, TCP—tissue culture plastic, RFU—relative fluorescence units. (b) Visualization of the fibroblasts viability during the first 3 days of growth on nonwovens. Live cells (green), dead cells (red). (c) Morphology of fibroblasts grown on nonwovens samples visualized by SEM and fluorescent microscopy; actin cytoskeleton (green) and nucleus (blue). Images were collected after 3 days of culture.