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Chromatogram of ultrafiltration-HPLC for screening aldose reductase (AR) inhibitors from the 70% MeOH extract of V. rigida root. (A) HPLC chromatography (254 nm) of the 70% MeOH extract of V. rigida root; (B) chromatograms of the centrifugal filtrates of AR-free group (black line) and AR group (blue line); (C) chromatograms of the centrifugal filtrates of AR group (blue line) and quercitrin-blocked AR group (red line). Compounds 1–7 were screened as AR inhibitors.
