Figure 2.

UV-vis photobleaching and acidification of the immunopurified ROS, WT, and RP mutant. Dark state (solid line), photobleached (dashed line), and acidified (dotted line). Samples in buffer B (137 mM NaCl, 2.7 mM KCl, 1.5 mM KH₂PO₄, and 8 Mm Na₂HPO₄, pH 7.4, 0.05% DM). Spectra were recorded at 20 °C.