Skip to main content
NCBI home page
Acta Veterinaria Scandinavica logoLink to Acta Veterinaria Scandinavica
. 2021 Jan 23;29(3-4):303–310. doi: 10.1186/BF03548622

Comparative Characterization and Biotyping of Staphylococcus aureus Isolates from Human and Bovine Sources

Sammenlignende beskrivelse og typebestemmelse af humane og bovine isolater af Staphylococcus aureus

Kaj Bruhn 1,✉, Ibrahim O Farah 1, Erik Pedersen 1, Christian Halgaard 1
PMCID: PMC8161648  PMID: 3076743

Abstract

One Hundred and ten alpha and/or delta-haemolytic isolates (collection 1), 50 beta haemolytic isolates (collection 2) from bovine mastitis, and 100 previously phage-typed alpha- and delta-haemolytic isolates (human collection) og Staphylococcus aureus (S. aureus) were tested and biotyped according to the scheme of Hajek & Marsalek (1971). Among collection 1 isolates, 85 (77.3 %) belonged to the human biotype A (human source). Twenty two (20 %) designated as non-allotted strains, possessed characteristics of both animal and human sources. The remaining 3 isolates (2.7 %) in this collection belonged to biotype C (animal source).

All collection 2 isolates which were used as control strains for animal sources, belonged to biotype C.

The human collection that contained 100 phage-typed haemolytic isolates (representing all human phage groups) were used as a control for the human source. Irrespective of their phage group, these strains predominantly produced alpha and/or delta haemolysins and belonged to the human biotype A. This study also recommended the use of a combined plasma crystal violet agar medium for the presumptive identification of S. aureus biotypes.

Keywords: mastitis, bacteriophage, non-allotted strains.

Full Text

The Full Text of this article is available as a PDF (1.8 MB).

Acknowledgments

This work was supported by FAO/DANIDA under the administration of the Development Cooperation Bureau, Copenhagen, Denmark. We are very grateful to Dr. K. Rosendal of the Department of Hospital Infections, SSI, Denmark, for phage-typing the bovine mastitis isolates and providing the phage-typed human strains.

References

  1. Adekeye D. Enterotoxin production by strains of Staphylococcus aureus isolated from animals and man in Nigeria. Vet. Microbiol. 1980;5:143–150. doi: 10.1016/0378-1135(80)90018-8. [DOI] [Google Scholar]
  2. Baird-Parker AC. The classification of Staphylococci and Micrococci from world-wide sources. J. gen. Microbiol. 1965;38:363–387. doi: 10.1099/00221287-38-3-363. [DOI] [PubMed] [Google Scholar]
  3. Blair JE, Williams REO. Phage-typing of Staphylococci. Bull. Wld. Hlth. Org. 1961;24:777–784. [PMC free article] [PubMed] [Google Scholar]
  4. Christie R, Wilson H. A test for staphylococcal fibrinolysin. Austr. J. exp. Biol. 1941;19:329. doi: 10.1038/icb.1941.49. [DOI] [Google Scholar]
  5. Cruickshank R, Duguid JP, Mamion BP, Swain PHA: Medical Microbiology 12th ed. Vol. 2. The practice of Medical Microbiology. Churchill Livingstone, Edingburgh, London and New York 1975.
  6. Devriese LA, Oeding P. Characteristics of Staphylococcus aureus strains isolated from different animal species. Res. Vet. Sci. 1976;21:284–291. doi: 10.1016/S0034-5288(18)33337-X. [DOI] [PubMed] [Google Scholar]
  7. Devriese LA. A simplified system for biotyping Staphylococcus aureus strains isolated from different animal species. J. appl. bacterid. 1984;56:215–220. doi: 10.1111/j.1365-2672.1984.tb01341.x. [DOI] [PubMed] [Google Scholar]
  8. Elias B, Kofer J. Staphylococcus aureus haemo-lysins their use in strain typing. Acta Microbiol. Acad. Sci. Hungary. 1980;27:183–190. [PubMed] [Google Scholar]
  9. Hajek V, Marsalek E. A study of Staphylococci of bovine origin. Staphylococcus aureus. 1972;26:154–160. [PubMed] [Google Scholar]
  10. Hajek V, Marsalek E. The differentiation of pato-genic staphylococci and a suggestion for their taxonomic classification. Zbt. Bakt. I. Abt. Orig. A. 1971;217:176–182. [PubMed] [Google Scholar]
  11. Israel L: In The Staphylococci. Cohen JO (Ed.) page 443–456. Willey-Interscience. New York-London-Sydney-Toronto 1972, pp 443–456.
  12. Janzen J J. Economic losses resulting from mastitis. J. Dairy Sci. 1970;53:1151–1161. doi: 10.3168/jds.S0022-0302(70)86361-5. [DOI] [PubMed] [Google Scholar]
  13. Klastrup O. Crystalviolet agar for the differentiation of staphylococci isolated from aseptically drawn milk samples. Preliminary communication. Nord. Vet. Med. 1955;7:315–320. [Google Scholar]
  14. Marsalek E, Hajek V. The classification of pathogenic staphylococci. Contributions to microbiology and immunology. 1973;1:30–37. [PubMed] [Google Scholar]
  15. Meyer W. A proposal for subdividing the species of Staphylococcus aureus. Int. J. Syst. Bacte-riol. 1967;77:387–389. doi: 10.1099/00207713-17-4-387. [DOI] [Google Scholar]
  16. Miljkovik V, Ranisavljevic M, Stojanovic L, Mija-cevic Z. Some characteristics and interaction of staphylococcal haemolysis isolated from cow milk. Acta Veterinaria (Beograd) 1974;24(2):42–53. [Google Scholar]
  17. Mohan K, Pal SC. Reciprocal transmission of Staphylococcus aureus between man and animals. J. Communicable Diseases. 1973;4:186–189. [Google Scholar]
  18. Rische H, Meyer W, Tschape W, Zome VD, Hummel R. The taxonomy of Staphylococcus aureus. Contributions to microbiology and immunology. 1973;1:24–29. [PubMed] [Google Scholar]
  19. Subcommunitee on Taxonomy of Staphylococci and Micrococci. Recommendations. Int. Bull. Bact. Taxonomy & Nomenclature 1965, 15, 109–110.
  20. Witte W, Grigorova M, Bajljosov D, Hummel R, Koruko VG. Ecology og Staphylococcus aureus. Comparative characterization of strains isolated from man, cat, sheep in Bulgaria and German Democratic Republic. J. Hyg. Epid. Microbiol, and Immunol. 1978;22(2):177–183. [PubMed] [Google Scholar]

Articles from Acta Veterinaria Scandinavica are provided here courtesy of BMC

RESOURCES