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. 2020 Apr 30;106(6):1659–1670. doi: 10.3324/haematol.2019.233924

Figure 5.

Figure 5.

E2 and E4 enhance human engraftment in immunodeficient male mice. (A) Experimental scheme of transplantation of human hematopoietic stem and progenitor cells (HSPC) into immunodeficient mice. Sublethally irradiated NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice were transplanted with human umbilical cord blood CD34+ (CBCD34+) cells, and 3 days later the animals were treated with vehicle or with daily low doses of either E2 or E4 (2 g of estrogen per day) for 4 days. Four months after transplantation, human hematopoietic engraftment was evaluated in the mouse bone marrow. (B) Percentage of hCD45+ cells in the bone marrow (BM) of male mice transplanted with 5x104 hCB-CD34+ cells 4 months after transplantation. (C) Percentage of hCD34+ cells within the human population in the BM of male mice transplanted with 5x104 hCB-CD34+ cells 4 months after transplantation. (D) Percentage of hCD34+hCD38- within the human population in the BM of the male mice transplanted with 5x104 hCB-CD34+ cells. (E) Percentage of human engraftment (hCD45+) in the BM of secondary NSG mice transplanted with 1x106 sorted hCD45+ cells from the primary recipients and analyzed 4 months after transplantation. Data were obtained from four biological replicates and are presented by dots and box-plots that represent the interquartile range (p75, upper edge; p25, lower edge; p50, midline; p95, line above the box; and p5, line below the box). Statistical significance was analyzed by the Mann-Whitney U test: **P<0.01 and ****P<0.001.